Which is the safer method for trophectoderm biopsy in mouse blastocyst, mechanical or laser?

Abstract

Which is the safer method for trophectoderm biopsy in mouse blastocyst, mechanical or laser? M.S. Jo, H.J. Lee, Y.J. Lee, S.C. Kim, J.K. Joo, K.S. LeeDepartment of Obstetrics and Gynecology, Pusan National University School of Medicine; Biomedical Research Institute, Pusan National University Hospital, Busan (Korea) Summary Introduction: This study was conducted to compare hatching rates after assisted hatching, re-expansion rates after trophectoderm biopsy, and survival rates after cryopreservation using different methods of assisted hatching and biopsy in mouse embryo. Materialsand Methods: Five-week-old female mice (C57BL/CBA) were superovulated, and two-cell embryos were collected. All embryos were cultured to blastocyst stage. For assisted hatching and separating trophectoderm from blastocyst, laser device and hand-made pipette were used respectively. Hatching rates after assisted hatching, re-expansion rates after trophectoderm biopsy, and survival rates after cry- opreservation were calculated. Results: Hatching rate was 92% in mechanically assisted hatching group and 90% in laser group, re- spectively. After mechanically assisted hatching, re-expansion rate was 91.3% and survival rate was 87% in biopsy by pipette and laser group, respectively. In laser hatching group, re-expansion rate was 88.9% with biopsy by pipette and survival rate was 84.4% with biopsy by laser. Conclusion: Throughout the study, mechanical technique and laser technique showed no differences in the safety pro- files in trophectoderm biopsy procedure. Key words: Trophectoderm; Preimplantation genetic diagnosis; IVF; Laser. Introduction Preimplantation