Age specific reference intervals of serum anti-Müllerian hormone concentration of 1,253 women with healthy females in infertility center

Abstract

Age specific reference intervals of serum anti-Müllerian hormone concentration of 1,253 women with healthy females in infertility center L.-S. Wang1, X.-L. Zhao1, X.-A. Cai1, G.-F. Fu1 1Department of Clinical Laboratory, The 105th Hospital of People Liberation Army, Hefei, Anhui Province (China) Summary Objective: To investigate the age specific reference intervals of serum anti-Müllerian hormone (AMH) concentration of healthy women in infertility center. Materials and Methods: After exclusion of 642 participator who did not meet the inclusion criteria, a total of 1,253 healthy women aged 17 to 49 years were enrolled in the study between January and August 2016. This study population was divided to five age groups, The samples of this study collected from each participants and aliquoted and stored at -80°C refrigeratory until assayed, The authors used AMH enzyme-linked immunosorbent assay (ELISA) kit for the assessment of AMH levels. Results: The serum AMH levels varied inversely correlation with patient age. The AMH concentration can be roughly calculated by formula (AMH = -0.327*age+15.98). This downward trend was significant among these women whom 40 years age. The coefficient of variation was larger even in the same age groups, The 2.5-97.5 th percentile of AMH level of each age group was 2.30-16.7 ng/mL, 0.96- 16.4ng/mL, 0.44-15.1ng/ mL, 0.14-12.5ng/mL, and 0.08-6.56ng/mL, respectively. Conclusions: This study determined reference values of serum AMH in China women with regular menstruation. These values can be applied to clinical evaluation and treatment of infertile women. It suggested that AMH is an individual medical index that women should establish their own baseline date for longitudinal axis comparison. Key words: Anti-Müllerian hormone; Reference intervals; Ovarian reserve.