Effects of selective agonists of adenosine, P2X, and P2Y receptors on motility of isolated fallopian tube

Abstract

Effects of selective agonists of adenosine, P2X, and P2Y receptors on motility of isolated fallopian tube M. Novakovic1, S. V. Jankovic1, I. Stojic1, J. Djuric, M. Folic, N. Folic1, A. Dimitrijevic1, G. Babic1, D. Stojadinovic1, S. M. Jankovic1 1Faculty of Medical Sciences, University of Kragujevac, Kragujevac (Serbia) Summary Background: During reproductive life of women, adenosine causes both contraction (with low concentrations) of fallopian tubes and inhibition of their spontaneous motor activity (with high concentrations). Objective: The aim of this study was to investigate effects of natural agonists of adenosine, P2X and P2Y receptors on motility of isolated fallopian tubes taken from postmenopausal women. Materials and Methods: Isolated preparations of isthmus and ampoule were made from fallopian tubes of 21 women in post-menopause, and then tested for reactivity on increasing concentrations of adenosine and P2X/P2Y selective agonists. Results: Adenosine showed concentration-dependent inhibitory effect on spontaneous contraction of both isthmic and ampullary segments of fallopian tubes, while P2X and P2Y agonists (adenosine-5-diphosphate, adenosine-5-triphosphate, uridine-5-diphosphate, and uridine-5-triphosphate) did not influence motility of the isolated preparations. Contractile effect of adenosine was not observed throughout the concentration range used in the experiments. Conclusions: Fallopian tubes of postmenopausal women are unresponsive to P2X and P2Y agonists, unlike those of women in reproductive period. Only an inhibitory effect of adenosine on spontaneous contractions of fallopian tubes is maintained in post-menopause, while a contractile effect is observed in younger women at low concentrations is lost. Key words: Adenosine; Fallopian tubes; Spontaneous contractions; P2X agonists; P2Y agonists.