Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/1956
Title: Morphometric image analysis of placental C-type lectin domain family 2, member D (CLEC2D) immuno-expression in HIV associated pre-eclampsia
Authors: Varaden, Deneshree
Keywords: Pre-eclampsia HIV LLT1 OCIL CLEC Preeclampsia Hypertension Pregnancy
Issue Date: Jul-2019
Abstract: Morphometric image analysis of placental C-type lectin domain family 2, member D (CLEC2D) immuno-expression in HIV associated pre-eclampsia Deneshree Varadena,*, Jagidesa Moodleyb, Onankoy A. Onyangungaa, Thajasvarie Naickera a Optics and Imaging Centre, University of KwaZulu-Natal, Durban, KwaZulu-Natal, South Africa bWomens Health and HIV Research Unit, University of KwaZulu-Natal, Durban, KwaZulu-Natal, South Africa A R T I C L E I N F O Article history: Received 25 November 2018 Received in revised form 11 April 2019 Accepted 29 April 2019 Available online 8 May 2019 Keywords: CLEC2D Pre-eclampsia HIV LLT1 OCIL CLEC Preeclampsia Hypertension Pregnancy A B S T R A C T Objective: C-type lectin domain family 2, member D (CLEC2D) is implicated in the immune response. Preeclampsia and HIV infection have opposing immune responses. In view of the high prevalence of HIV infection and pre-eclampsia in South Africa, this study assessed the placental immuno-expression of CLEC2D in HIV associated pre-eclampsia. Method: Placental tissue was obtained from 60 pregnancies which were categorized according to pregnancy type (pre-eclamptic or normotensive) and HIV status (positive or negative). Immunohistochemistry and morphometric image analysis were used to evaluate placental CLEC2D immunoexpression. Results: CLEC2D expression was significantly decreased in the conducting villi of pre-eclamptic vs normotensive placentae (p = 0.0418) but was increased in the exchange villi, albeit non-significant (p = 0.4948). HIV positive status intensified placental CLEC2D immuno-expression in conducting (p = 0.0312) and exchange (p = 0.0025) villi. CLEC2D expression was significantly different in exchange vs conducting villi (p < 0.0001) and across study groups (p = 0.0003). Normotensive; HIV negative placentae (control) had a non-significant difference in CLEC2D expression across villi types, however significant difference was noted within the remaining groups: normotensive; HIV positive (p < 0.05), pre-eclamptic; HIV positive (p < 0.01 and pre-eclamptic; HIV negative (p < 0.001). Conclusion: The contrasting expression of CLEC2D in HIV infection and pre-eclampsia is demonstrative of the immunosuppressive and pro-inflammatory roles of the respective pathologies. However, this implication may be confounded by highly active anti-retroviral treatment (HAART).
URI: http://localhost:8080/xmlui/handle/123456789/1956
Appears in Collections:1. European Journal of Obstetrics & Gynecology and Reproductive Biology

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