Antioxidant Activity of Senna (Senna alexandrina MILL.) Leaf Extracts

Abstract

Senna leaf plant (Senna alexandrina Mill.) is empirically effective in treating several diseases. Senna leaves contain saponins, alkaloids, glycosides, flavonoids, phenols, sesquiterpenes, tannins, and phytosterols. This study aims to assess Senna leaf extract's antioxidant activity, flavonoid, and phenolic content (Senna alexandrina Mill.) with various extraction methods and solvent variations. Senna (Senna alexandrina Mill.) leaves were extracted using Ethanol 96%, methanol, and ethyl acetate by maceration and ultrasonic methods. The extracts obtained were then tested for antioxidant activity and to determine the phenolic and flavonoid content using UV-Vis spectrophotometry. The highest total phenolic content obtained from maceration and ultrasonic extraction methods were ethanol extracts, with 12.96 and 14.08%. The highest total flavonoid content obtained from maceration and ultrasonic extraction methods was from ethyl acetate extract, with the same level of 7.4%. In conclusion, the highest phenolic and flavonoid content were obtained by ultrasonic extraction 14.08%. and 7.4%, respectively. Then, the reducing radical DPPH showed the potential antioxidant IC50 119.9 𝝁g/mL was collected by using UAE.