Histological evaluation of effects of krill oil and eucalyptus oil on bone healing in rats

Abstract

Background: Bone defect repair is a significant challenge in orthopedic medicine. Objectives: This study assessed the effectiveness of applying krill oil locally as an osteoinducer in bone healing, and the impact of eucalyptus oil as a stimulant for healing bone defects. Subjects and methods: In this study, 48 albino male rats weighing 300-400 g and aged 6-8 months were used, where their environment was carefully monitored, including the amounts of water and food consumed. The experimental test site was the side of the right tibia and the control site was on the left tibia. The treatment was performed on the inner side of the tibia. Bone deficit treatment on the control site involved applying 1 mL of distilled water topically once each day. The experimental treatments were tested in three subgroups: group I received daily local application with 1 mL of krill oil, group II received daily local application with 1 mL of eucalyptus oil, and group III received daily local application with 1 mL of-a combination of krill oil and eucalyptus oil. Six rats were sacrificed at 7 and 14 days after surgery, with 12 rats in each group. Results: Osteoid tissue development and dramatic increases in the numbers of bone cells were observed after local administration of either krill oil or eucalyptus oil separately, or both substances combined, to a tibia lesion. Notable changes in the area of bone marrow occurred, with increases in the trabecular bone area and gradual decreases in the trabecular bone number. Conclusion: Combined treatment with krill oil and eucalyptus oil has therapeutic potential for accelerating the mending of bone deformities. Krill and eucalyptus oils can affect osteogenesis and angiogenesis processes.