Serum Gel Formulation of Mundu Fruit Extract (Garcinia dulcis Roxb. Kurz.) as Antioxidant and Anti-elastase

Abstract

UV radiation can generate Reactive oxygen species (ROS), thereby damaging neutrophils, leading to the uncontrolled release of elastase, and causing aging. This study aims to examine the antioxidants and anti-elastase activity of the Garcinia dulcis Roxb-Kurz (Mundu) fruit and in the serum gel extract preparation. Extraction of Garcinia dulcis was carried out using microwave-assisted extraction (MAE) using ethanol, and the antioxidants activity test was done using the ABTS (2,2’-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) method. Preparation of serum gel uses three formulas (F1, F2, and F3) that contained extract ethanol of Garcinia dulcis with the concentrations 100x, 200x, and 300 x IC50 value, consecutively. The test results for the three formulas showed the IC50 of antioxidants were 88.61, 71.47, and 66.26 μg/ml. The anti-elastase activity of serum gel obtained 150.32, 134.47, and 111.48 μg/ml, respectively. This shows that the best activity is the F3 of serum gel, indicating that the higher the extract concentration in the formulation provides better activity in the serum gel preparation. In addition, evaluation of serum gel for three formulas were carried out, including pH, viscosity, and spreadability, and F3 was the best formula. In conclusion, the Mundu fruit extract has antioxidant and anti-elastase activity and, after being applied to serum gel preparations, still provides good antioxidant and anti-elastase activity results. Keywords: Garcinia dulcis Roxb-Kurz extract, antioxidants; anti-elastase; formula; gel.