http://localhost:8080/xmlui/handle/123456789/9725 1 - 156 Wed, 15 Apr 2026 15:07:18 GMT 2026-04-15T15:07:18Z http://localhost:8080/xmlui/handle/123456789/9745 Title: Unveiling unique effector function-related bulk antibody profiles in long-term hemodialysis patients following COVID-19 mRNA booster vaccination Authors: Chou, Chia-Yi; Cheng, Chung-Yi; Lee, Chih-Hsin; Kuro-O, Makoto; Chen, Tso-Hsiao; Wang, San-Yuan Abstract: Background: Hemodialysis patients exhibit a reduced response to vaccination and have different vaccine dose regimens. Vaccines induce antibodies and affect the inflammatory balance through antibody glycosylation and effector functions. Therefore, we aimed to analyze the antibody glycosylation profiles in hemodialysis patients who were vaccinated against severe acute respiratory syndrome coronavirus 2, infected with the virus, or both, and compare them with those of dialysis patients in a control group. Methods: Plasma samples from 112 hemodialysis patients were assigned to four groups: control, infected, vaccinated, and post-vaccine-infected. Paired plasma samples from 47 people with vaccination (vaccinees) were analyzed before and after the booster dose. The same analytical approach was applied to the four groups for a cross-sectional comparison. Results: Our study found that both vaccination and infection groups showed decreased fucosylation of IgG1, which is associated with a proinflammatory biosignature. However, vaccination also leads to increased galactosylation and bisection of IgG antibodies, which are associated with anti-inflammatory effects and the additional regulation of immune responses. In contrast, infection led to an additional decrease in the fucosylation of IgG2 and IgA, demonstrating a more intense proinflammatory biosignature than vaccination. Conclusions: Our findings emphasize the proinflammatory biosignature of afucosylation in both vaccination and infection groups. Additionally, we uncovered further regulated profiles related to galactosylation in vaccinees. These findings suggest that antibody investigation for vaccination or infection should not solely focus on neutralization but should also consider effector function-related glycosylation profiling. This comprehensive information can be valuable for fine-tuning vaccine development in the future. Sat, 01 Feb 2025 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/9745 2025-02-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/9744 Title: Signatures of lower respiratory tract microbiome in children with severe community-acquired pneumonia using shotgun metagenomic sequencing Authors: Yen, Ting-Yu; Hsu, Ching; Lee, Ni-Chung; Wu, Chao-Szu; Wang, Hsin; Lee, Kuan-Yi Abstract: Background: Severe community-acquired pneumonia was associated with high morbidity and mortality in children. However, species-level microbiome of lower airway was sparse, and we used shotgun metagenomic nextgeneration sequencing to explore microbial signatures. Methods: We conducted a prospective cohort study to recruit children under 18 who required admission to an intensive care unit for community-acquired pneumonia between December 2019 and February 2022. Lower respiratory specimens were collected on admission for shotgun metagenomic sequencing. The children were divided into two groups. Critical cases were patients with respiratory failure requiring endotracheal ventilator support, and severe cases did not require intubation. Signatures of lower respiratory tract microbiome were compared between groups using an exact k-mer matching metagenomic analysis pipeline (Kraken 2) and a metagenome-assembled genomes pipeline (MetaWRAP). Results: Totally 66 children were enrolled, and 27 children were critical cases, and the rest were severe cases. There were significant differences in microbial community structure between different severity groups, and microbial abundance was negatively correlated with disease severity. The results showed that Haemophilus influenzae was more prominent in children who were critical, accompanied with increased expression of intracellular transport, secretion, and vesicle transport genes. Rothia mucilaginosa, Dolosigranulum pigrum, and Prevotella melaninogenica tended to be present in less severe community-acquired pneumonia group. Conclusion: This study demonstrated that significantly different microbial community was associated with severity of community-acquired pneumonia requiring intensive care admission. Species-level shotgun metagenomic sequencing facilitates the exploration of potentially pathogenic or protective microbes and shed the light of probiotic development in lower respiratory tract. Sat, 01 Feb 2025 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/9744 2025-02-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/9743 Title: Serum Mpox-specific IgG titers before and after breakthrough Mpox infection in an HIV-infected individual with viral suppression and prior 2-dose Mpox vaccination Sat, 01 Feb 2025 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/9743 2025-02-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/9742 Title: Recommendation for the use of respiratory syncytial virus vaccines Authors: Lee, Ping-Ing; Huang, Yhu-Chering; CLiu, Ching-Chuan; Chen, Shee-Uan; Hsueh, Po-Ren; Ku, Shih-Chi Abstract: Respiratory syncytial virus (RSV) is the most common pathogen for young children hospitalized with bronchiolitis and pneumonia. Most infections occur below 1 year of age. RSV is also a significant viral pathogen for adults with respiratory tract infection. Vaccines targeting the pre-fusion protein of RSV, including recombinant and mRNA vaccines, are now available. A committee of experts from related fields was convened by the Taiwan Immunization Vision and Strategy to develop recommendations for RSV vaccination in the elderly and pregnant women. The recommendation is not intended as a sole source of guidance in the prevention of RSV infection in children. The provisions listed in this recommendation are comprehensive suggestions made by experts in Taiwan based on existing medical evidence. This recommendation should be subject to modification in light of additional medical research findings in the future, and these provisions should not be cited as a basis for dispute resolution. Sat, 01 Feb 2025 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/9742 2025-02-01T00:00:00Z