DSpace Collection: 77 - 124

Sigma metric evaluation with different TEa targets in clinical biochemistry

Sat, 01 Jan 2022 00:00:00 GMT

Title: Sigma metric evaluation with different TEa targets in clinical biochemistry Authors: Korkmaz, Seniz Abstract: Objectives: The aim of this study was to evaluate the analytical performances of various clinical biochemistry analytes by the sigma metrics method according to different total allowable error (TEa) targets and to determine the causes of errors that lead to low sigma score by using Quality Goal Index (QGI). Methods: The study was carried out in the Central Laboratory of Bursa Karacabey State Hospital. Twelve analytes that were studied on the Roche Cobas c 501 autoanalyzer were included in the study. Internal (level 1 and 2) and external quality control data for the period March–August 2020 were obtained retrospectively. The TEa targets were obtained from the Clinical Laboratory Improvement of 2019 (CLIA 2019), biological variation database (BVD), Rili-BAEK, and Turkish data. QGI was calculated for analytes with sigma score <3 according to CLIA. Results: According to the TEa goals of four different guides, different sigma scores were obtained. Three parameters with sigma scores <3 were determined according to TEa targets of CLIA, 8 according to BVD, and 6 according to Rili- BAEK, while there were no parameters with sigma score <3 according to the TEa targets of Turkey. Number of parameters with sigma scores >6 were 7, 10, 6, and 18 according to TEa targets of CLIA, BVD, Rili-BAEK, and Turkey, respectively. When QGI was calculated, it was found that there was inaccuracy problem for albumin and chlorine L1 and imprecision for chlorine L2. Conclusion: Laboratories should determine the appropriate TEa targets and use the sigma metrics method and QGI as a quality improvement tool. In the light of the obtained data, necessary quality improvements should be made, and the reliability of the results should be increased.

Comparison of Diagon Coag XL and Cobas t 511 analyzers

Sat, 01 Jan 2022 00:00:00 GMT

Title: Comparison of Diagon Coag XL and Cobas t 511 analyzers Authors: Guven, Berrak; Benice, Ismail; Can, Murat Abstract: Objectives: We compared prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen, and Ddimer test results measured using the Diagon Coag XL coagulation analyzer with Cobas t 511 analyzer. Methods: Imprecision studies were performed for the PT, aPTT, fibrinogen, and D-dimer tests used by the Diagon Coag XL analyzer. For the comparison study, we used the leftover citrated plasma from patient samples after routine analysis with Cobas t 511. All of the results were analyzed using the correlation coefficient and Passing–Bablok regression analysis. Results: Total coefficient of variation obtained for all tests were within the criteria for acceptance. The method comparison study showed a good correlation between the results obtained on Diagon Coag XL and Cobas t 511 analyzers, except for aPTT test. The correlation coefficients obtained were 0.98 for PT, INR, and D-dimer, 0.95 for fibrinogen, and 0.80 for aPTT. Conclusion: For PT, aPTT, fibrinogen, and D-dimer tests, Diagon Coag XL analyzer is suitable for monitoring the coagulation system, and it can be used in clinical laboratories. However, the precision values of tests stated by the manufacturer must be verified.

Evaluation of oxidative stress in gout disease; thiol-disulfide homeostasis and ischemia-modified albumin levels

Sat, 01 Jan 2022 00:00:00 GMT

Title: Evaluation of oxidative stress in gout disease; thiol-disulfide homeostasis and ischemia-modified albumin levels Authors: Balik, Ahmet Rifat; Omma, Ahmet; Sandikci, Sevinc Can; dkk. Abstract: Objectives: Gout is a common and easily treated disease characterized by the accumulation of monosodium urate crystals in both joints and other tissues. Monosodium urate crystals are the main stimulants for initiating and maintaining an inflammatory response. Oxidative stress is also an early change in gout pathogenesis together with inflammation. This study aimed to investigate the presence of oxidative stress in gout together with thiol–disulfide homeostasis and ischemia- modified albumin (IMA) levels. We aimed to compare these parameters with inflammation marker C-reactive protein (CRP) and high-sensitivity C-reactive protein (hsCRP). Methods: Levels of native thiol, total thiol, disulfide, IMA, CRP, and hsCRP were detected in patients with gout (n=50) and healthy subjects (n=50). Student’s t-test and the Mann-Whitney U test were used for istatistical analysis. Results: Native thiol, total thiol, and index 3 (native thiol/total thiol×100) were significantly lower in the patient group, while disulfide, index 1 (disulfide/native thiol×100), index 2 disulfide/total thiol×100), IMA, CRP, and hsCRP were significantly higher. In addition, elevation in native thiol, total thiol, and disulfide levels was detected as disease duration increased. Conclusion: The present study has shown the role of oxidant damage in gout disease. Additional studies are needed to identify sources of oxidative stress in gout.

Intra-day changes in the levels of biochemistry parameters

Sat, 01 Jan 2022 00:00:00 GMT

Title: Intra-day changes in the levels of biochemistry parameters Authors: Arpaci, Abdullah; Dogan, Serdar; Gul, Bahar Unlu; dkk. Abstract: Objectives: People mostly live in the nonfasting state during a normal 24-h cycle. This study aims to compare the levels of 18 biochemical parameters during different hours of the day. Methods: A total of 18 biochemical tests of patients who visited outpatient clinics only once between January 1, 2010, and December 31, 2019, were evaluated at the Hatay Mustafa Kemal University (HMKU) Central Laboratory by using hospital database information. The tests are albumin (Alb), aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), amylase, blood urea nitrogen (BUN), calcium (Ca), total cholesterol (TC), creatine kinase (CK), creatinine (Cr), gamma-glutamyltransferase (GGT), glucose, high-density lipoprotein cholesterol (HDL-C), inorganic phosphorus (Pi), iron (Fe), total protein (TP), triglyceride (TG), and lipase. The blood samples of the patient were divided into eight groups according to their collection time as follows: (a) 07:00-07:59, (b) 08:00-08:59, (c) 09:00-09:59, (d) 10:00-10:59, (e) 11:00-11:59, (f ) 12:00-13:59, (g) 14:00-14:59, and (h) 15:00-17:00. Results: A statistically significant difference was found between the groups in terms of all parameters except amylase, GGT, and TP (p<0.05). The effect size refers to the minimum amount of difference that is clinically significant. According to the effect size values, there was no significant difference between time groups in the following parameters: Alb, ALT, AST, Pi, Ca, TC, Cr, Fe, glucose, BUN, lipase, TG, ALP, HDL-C, and CK (ʈ<0.30). Conclusion: When considering all of the results, nonfasting screening would not only be acceptable but also make physiologic sense.