DSpace Collection: 143 - 215

Impact of the presence of debris on semen analysis using an automated system (SQA-V)

Fri, 01 Jan 2021 00:00:00 GMT

Title: Impact of the presence of debris on semen analysis using an automated system (SQA-V) Authors: Ilardo, Claudio; Ostengo, Violaine; Eon, Leatitia; dkk. Abstract: Objectives: This study examined the influence of the manual assessment of debris on the results of sperm concentration, progressive motility, and normal morphology measured using the SQA-V system (Medical Electronic Systems LLC, Los Angeles, CA, USA). Methods: Sixty samples were analyzed simultaneously and independently by different operators using the manual technique and the SQA-V analyzer. Three measurements (sperm concentration, progressive motility, and normal morphology) of each sample were assessed using the 4 debris assessment levels: none/few, moderate, many, and gross. Results: Given an optimal assessment of debris, the study data indicate that the SQA-V provided results that had very good agreement with those of the manual method: sperm concentration (rho=0.987, regression analysis formula: y=0.961x+1.962, p<0.0001), progressive motility (rho=0.949, regression analysis formula: y=0.989x+0.418, p<0.0001), and normal morphology (rho=0.694, regression analysis formula: y=0.678x+4.061, p<0.0001). Underestimation of debris increased sperm concentration and decreased motility and normal morphology, while overestimation of debris decreased sperm concentration and increased motility and normal morphology. Conclusion: The results indicated that the performance of the SQA-V remains subject to the competency of the operator.

Erroneously high troponin measurement caused by fibrin clot: Two cases

Fri, 01 Jan 2021 00:00:00 GMT

Title: Erroneously high troponin measurement caused by fibrin clot: Two cases Authors: Yavuz, Hatice Bozkurt Abstract: Acute myocardial infarction is the most common cause of morbidity and mortality in the world. Cardiac troponin measurements play a key role in the diagnosis. However, preanalytical errors as a result of the presence of fibrin or interference due to conditions such as heterophile antibody positivity may cause erroneously high results. Such errors may result in invasive procedures, such as angiography, which may add unnecessary risk. In our hospital, high-sensitivity troponin- I (hs-TnI) was routinely analyzed using a serum separator tube (reference value: female <15.5 pg/mL, male <34.2 pg/mL). This report describes the cases of 2 patients with a false initial troponin measurement: a 19-year-old male patient and a 55-year-old female patient. The hs-TnI value of the male patient was initially measured as 55.5 pg/mL. After the analysis, it was noted that the sample contained fibrin. The sample was centrifuged again and the TnI result was 1.8 pg/mL. Similarly, the TnI result of the female patient was first measured as 90.2 pg/mL. When it was observed that there was fibrin present, the sample was recentrifuged. The revised result was 2.4 pg/mL. The laboratory staff were trained on preanalytical errors, and the use of lithium heparin tubes was implemented in the laboratory as an additional means to eliminate the problem of fibrin interference.

Severe methemoglobinemia caused by prilocaine: A rare case report

Fri, 01 Jan 2021 00:00:00 GMT

Title: Severe methemoglobinemia caused by prilocaine: A rare case report Authors: Karabulut, Alpaslan; Sahin, Mustafa Abstract: Hemoglobin is a molecule found in erythrocytes that transports oxygen to tissues. Methemoglobin, a form of hemoglobin that can no longer bind oxygen, is formed when the ferrous iron in normal hemoglobin becomes ferric iron as a result of oxidation due to various kinds of oxidative stress. Methemoglobinemia can be congenital, however, exposure to toxins is more often the cause. Local anesthetics can occasionally cause methemoglobinemia, and the potential effects increase with combined or excessive use. Prilocaine-induced methemoglobinemia has been reported, though it is rare. Presently described is the case of an adult case of methemoglobinemia, which developed following a prilocaine injection for fibromyalgia pain. Methylene blue treatment and adjuvant therapy resulted in recovery. This case report serves as a reminder that that prilocaine can be a cause of adult-acquired methemoglobinemia. Methylene blue treatment and adjuvant therapy consisting of infusions of 100% oxygen and 0.9% sodium chloride administered promptly can prevent potentially severe toxic effects.

Flow cytometric evaluation of cancer stem cell markers in HepG2 cells following sorafenib treatment

Fri, 01 Jan 2021 00:00:00 GMT

Title: Flow cytometric evaluation of cancer stem cell markers in HepG2 cells following sorafenib treatment Authors: Cakil, Yaprak Donmez; Sitar, Mustafa Erinc; Ozunal, Zeynep Gunes; dkk. Abstract: Objectives: Liver cancer is a leading cause of mortality. Sorafenib resistance and cancer stem cells (CSCs) are among the factors that contribute to a poor prognosis. Different drugs enrich different CSC populations with a variety of CSC markers. This study investigated the expression of CSC markers in HepG2 cells in response to low doses of sorafenib using flow cytometry. Methods: The cytotoxicity of sorafenib was determined using a cell counting kit-8 assay. The expressions of the CSC markers CD44, CD90, and CD133 were measured with flow cytometry after treatment with sorafenib for 72 hours. Results: Sofranib inhibited cell proliferation in a dose-dependent manner. Low-dose sorafenib treatment increased CD44 expression; however, there was a decrease in the expression of CD133. An increasing trend was also seen in CD90 expression, but the difference was not significant. Conclusion: The results indicate that CSC expression varied according to the sorafenib dose administered, which supports the role of CSCs as novel pharmacological targets and highlights the importance of their characterization and the ability to identify them.