http://localhost:8080/xmlui/handle/123456789/7723 Thu, 09 Apr 2026 14:54:37 GMT 2026-04-09T14:54:37Z http://localhost:8080/xmlui/handle/123456789/7870 Title: DNA identification of kayu kuning (yellow-fruited moonseed) from East Kalimantan, Indonesia Authors: Sulistiarini, Riski Abstract: Kalimantan is an island with an abundance of kayu Kuning (yellow-fruited moonseed). Kayu kuning is used for three different plant species, namely Arcangelisia flava (L.) Merr., Fibraurea tinctoria Lour., and Coscinium fenestratum (Gaertn.) Colebr. Therefore, it creates confusion and may cause improper use. It has properties such as anti-diabetic, antiplasmodial, antidiarrheal, hepatitis, and antidote. The study uses the DNA barcode technique to identify kayu kuning (yellow-fruited moonseed) from East Kalimantan, Indonesia. The genomic DNA of kayu kuning (yellow-fruited moonseed) was extracted, and ITS primers were used for identification using polymerase chain reaction (PCR). It was compared with Arcangelisia flava, Fibraurea tinctoria, and Coscinium fenestratum as a phylogenetic tree. DNA sequence alignment of ITS and phylogenetic reconstruction revealed that kayu kuning (yellow-fruited moonseed) from East Kalimantan was closely related to A. flava. The kayu kuning (yellow-fruited moonseed) had 94.16% of sequence similarity with A. flava according to the ITS1 barcode. Tue, 01 Nov 2022 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/7870 2022-11-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7868 Title: Analysis of plasmid profiles of Escherichia coli bacteria and their resistance to several antibiotics Authors: Nurdevi, Aldise Indah; Marpaung, Mauritz Pandapotan Abstract: Escherichia coli is a bacteria belonging to the family Enterobacteriaceae. Escherichia coli is a harmful gram bacterium living in the digestive tract. These bacteria also live in water and soil, commonly referred to as coliform. This research aims to analyze the plasmid profile of Escherichia coli bacteria and its resistance to some antibiotics. To explore the plasmid profiles of Escherichia coli in a recent research electrophoresis gel, the antibiotic used in its resistance tests is the Amoxicillin, Tetracycline antibiotic, Ciprofloxacin, Sulfamethoxazole, and Streptomycin antibiotic. The electrophoresis results in a difference in the number of plasmid profiles with 1 to 2 ribbons of plasmid DNA different sizes from ˜1500 bp to ˜2300 bp. Antibiotic resistance tests have occurred in Amoxicillin, Ciprofloxacin, and some resistance samples to the Streptomycin antibiotic. Tue, 01 Nov 2022 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/7868 2022-11-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7867 Title: Antibacterial activity of methanol extract Rhizophora mucronata leaves toward Salmonella typhi: leading the typhoid fever Authors: Nur, Rinto Muhammad; Dewi, Resmila; Kaliu, Sutriani Abstract: The community has utilized mangrove extensively, particularly as a component of traditional medicine. Rhizophora mucronata is one species that possess antibacterial, antiviral, antifungal, and insecticidal properties. According to reports, R. mucronata has antibacterial properties against the Salmonella typhi bacteria that cause typhoid fever. This research aims to obtain a methanol extract of R. mucronata leaves and assess its antibacterial potential as natural new medicine, particularly for treating typhoid fever. The extraction method is maceration with a 70 percent methanol solvent. Well, diffusion is utilized to determine antibacterial activity. Alkaloid, flavonoid, saponin, steroid, tannin, and triterpenoid are the chemical compounds identified in the methanol extract of R. mucronata leaves (MERmL). Ten percent concentration of MERmL exhibited moderate antibacterial activity (7.97±0.25 mm), whereas 30 percent concentration (11.380.29 mm) and 50 percent concentration (16.07±0.40 mm) exhibited intense antibacterial activity. Based on these findings, R. mucronata leaf methanol extracts with higher concentrations have more potent antibacterial activity against S. typhi in typhoid fever treatments. Tue, 01 Nov 2022 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/7867 2022-11-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7866 Title: Fractionation of a phenolic compound from water spinach (Ipomoea aquatica) herbs as anti-dandruff against Malassezia sp. Authors: Damaharyuningtyas, Meta; Kintoko; Darmawan, Endang Abstract: Dandruff is a scalp disorder caused by a fungus (Malassezia sp.). Water spinach (Ipomoea aquatica) contains phenolic compounds which have antifungal activity. The purpose of this research is to know the active fraction of water spinach (Ipomoea aquatica) herbs that have an antidandruff activity against Malassezia sp. The study used a true experimental design for an antidandruff activity test. Fractionation of water spinach (Ipomoea aquatica) methanol extracts used column chromatography which stationary phase with silica gel 60 powder and mobile phase with chloroform: ethanol: acetyl acetate (8:2:0,1). In vitro anti-dandruff activity based on minimum inhibitory concentration (MIC) and minimum antifungal concentration (MFC) against Malassezia sp. of human dandruff isolate. The average of MIC and MFC among groups fraction of water spinach (Ipomoea aquatica) methanol extracts compared used Friedman test (p≤ 0,05). Fractionation produces 12 fractions, and fraction number 7 of water spinach (Ipomoea aquatica) herbs have the best anti-dandruff activity against Malassezia sp. with MIC 125 µg/mL and MFC 250 µg/mL identified as a phenolic compound. Based on the results, a fraction of phenolic compounds from water spinach (Ipomoea aquatica) herbs have anti-dandruff activity against Malassezia sp. Tue, 01 Nov 2022 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/7866 2022-11-01T00:00:00Z