DSpace Collection: 1217 - 1233

Citrus Extract-Mediated Zinc Oxide Nanoparticles and Their Capacity to Attenuate Free Radicals and Inflammation

Mon, 01 Jan 2024 00:00:00 GMT

Title: Citrus Extract-Mediated Zinc Oxide Nanoparticles and Their Capacity to Attenuate Free Radicals and Inflammation Authors: Diniso, Tabisa; Oriola, Ayodeji O.; Miya, Gugulethu M.; dkk. Abstract: Introduction: The exploitation of plant materials for the green synthesis of nanoparticles (NPs) for biological applications, is considered an eco-friendly technology because it does not involve the use of toxic chemicals. Objective: The study was carried out to synthesize citrus extract-mediated ZnO NPs and evaluate their free radical scavenging and anti-inflammatory capacity. Materials and Methods: ZnO NPs were green synthesized, using the peel and leaf aqueous extracts of three citrus plants: Nova mandarin, Satsuma mandarin and Eureka lemon. The citrus extract based ZnO NPs were characterized by UV-Vis and FTIR spectroscopy, microscopy (SEM and TEM), EDX and XRD analyses. They were screened against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) free radicals. Finally, their inhibitory effects against egg albumin denaturation (EAD) were determined spectrophotometrically. Results: The six afforded biogenic NPs consistently exhibited FTIR vibrational band around 500 cm-1, which is characteristics of a metal oxide (Zn-O) band. They also showed UV-Vis absorption peaks at 387 and 415 nm, suggesting the formation of ZnO NPs. Nova mandarin peel (NMP) ZnO NPs exhibited the best DPPH and NO radical scavenging activities, with 50% inhibitory concentration (IC50) of 7.61±0.69 and 19.93±0.40 μg/mL, respectively. It also gave the best inhibition against EAD, with an IC50 of 14.80±1.29 μg/mL. Morphological assessment of NMP extract-based ZnO NPs revealed rod-shaped particles at 35-50 nm range. Conclusion: It has been shown through this study that citrus extract based ZnO NPs, especially those prepared with NMP, may have the capacity to attenuate free radical release and inflammation in biological systems.

Evaluating the Efficacy of Cresvin beta on Type 2 Diabetes Mellitus Management: A Randomized Comparative Clinical Trial

Mon, 01 Jan 2024 00:00:00 GMT

Title: Evaluating the Efficacy of Cresvin beta on Type 2 Diabetes Mellitus Management: A Randomized Comparative Clinical Trial Authors: N, Nandakumar K; Nehru, Mohanraj; Subramanian, Prasanth; dkk. Abstract: Background: In this current state, Type 2 Diabetes Mellitus (T2DM) is more prevalent in the population, and metformin is used as a first-line medication for treating it, but gradually prevalence and incident rate of T2DM is increased. There was an upsurge in the utilization of alternative therapies in managing of diabetes. Especially in diabetes, Herbal medicines are considered safe and reliable by the majority of the population. This research aims to estimate the safety and efficacy of poly herbal metabolite compounds of Cresvin beta capsules in adults with T2DM. Methods: In this study, 60 T2DM patients aged 18-60 years were randomly assigned to Groups A (30), receiving Metformin 500 mg twice a day after food, and Group B (30), receiving Cresvin beta capsule 500 mg twice a day, after food in a prospective, randomized and open-label clinical study. The in-silico simulation study was performed on selected plants major compounds on target protein Insulin-like Growth Factor-1 (1K3A). Results: The Cresvin beta is found to be safe and effective in the management of T2DM. The FBS, PPBS and HbA1c were significantly lowered (p<0.001) in posttreatment in both the Metformin and Cresvin beta capsules. Similarly, the levels of IGF1, adiponectin, EL-1, IL-6, and TNF-α showed significant alteration (p<0.001) after the treatment. The alterations found in the post-treatment results of Cresvin beta, including the reduced levels of creatinine and triglycerides, express the efficacy. Conclusion: The research results conclude, that the Cresvin beta capsule would be one of the suitable choices for increasing the efficacy in the management of diabetes mellitus.

Evaluation of Anti-oxidant Activity and Flavonoid Content in Macaranga peltata Leaves

Mon, 01 Jan 2024 00:00:00 GMT

Title: Evaluation of Anti-oxidant Activity and Flavonoid Content in Macaranga peltata Leaves Authors: H, Honnesh N; Saha, Santanu Abstract: Introduction: Macaranga peltata is a woody shrub and tree found in forest of Western Ghats of India. The leaves of M. peltata are large so used to wrap sweets in kerala. The plant is used in folk medicine and siddha medicine to treat fever, cough, wound and ulcers. There is very limited research has been conducted on exploring this plant medicinal properties and phytochemical estimation. Materials and Methods: In the present study attempt was made to know the antioxidant activity of MPCF by DPPH assay, FRAP assay and H2O2 assay methods and Optimization of mobile phase by TLC method and supporting major flavonoid content quantification by HPTLC method Results: The result shown the Chloroform fraction has significant antioxidant activity of IC50 value of 123.71(DPPH assay), 185.85(FRAP assay) and 204.31(H2O2 assay). The TLC method optimum mobile phase was found to be Toluene: Ethyl acetate: Formic acid (5:4:0.2). The standard flavonoids used are Luteolin, Diosmin and ellagic acid. The HPTLC method results shown MPCF was found to contain 11.70% Luteolin.