http://localhost:8080/xmlui/handle/123456789/10370 Thu, 09 Apr 2026 10:02:01 GMT 2026-04-09T10:02:01Z http://localhost:8080/xmlui/handle/123456789/10421 Title: Cytotoxic Activities of Bioactive Fraction of Uncaria gambir (Hunter) Roxb on MCF-7 Human Breast Cancer Cell Line Authors: Yunarto, Nanang; Fadhilah, Humaira; Purnamasari, Rini; Nourma Reswandaru, Uud Abstract: Breast cancer is a disease with the highest mortality rate. The first line of breast cancer treatment uses chemotherapy, but it has serious side effects on patients. Gambir (Uncaria gambir (Hunter) Roxb.) contains catechin compounds that show cytotoxic activity. This study aims to evaluate the antioxidant activity and cytotoxic effects on MCF-7 human breast cancer cells from the bioactive fraction of gambir leaves. Gambir extract was fractionated with water, ethyl acetate, and n-hexane solvents. Determination of catechin levels in the fractions used high-performance thin-layer chromatography. Antioxidant activity was tested using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and cytotoxicity tests on MCF-7 cells were carried out using MTT assay with inhibition of protein expression anti-apoptosis was determined by immunocytochemistry assay. The ethyl acetate fraction had the highest catechin content, namely 93.05%. The results of the antioxidant activity test showed that the IC50 values were consecutively for the water fraction 42.48 μg/mL, ethyl acetate 14.86 μg/mL, and n-hexane 49.63 μg/mL. In the MTT test, the ethyl acetate fraction had more potent cytotoxic activity with an IC50 value of 87.52 μg/mL. This study shows that the ethyl acetate fraction of gambir leaves has strong antioxidant and cytotoxic activity. The higher catechin content relates to antioxidant and cytotoxic activity against MCF-7 cells Mon, 01 Jan 2024 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/10421 2024-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/10420 Title: Formulation of Komba-Komba Leaf Extract Ointment (Chromolaena odorata L) for Wound Healing Authors: Maulana Satria, Beny; Zulfahrin UZ, LM; Sopian, Ahmad Abstract: Wounds are physical injuries that can cause damage to skin tissue. The extract of komba-komba leaves (Chromolaena odorata L) contains flavonoids, saponins, and tannins, which are wound healers formulated as ointment preparations. This study aims to determine how administering komba-komba leaf extract ointment affects wound healing. The maceration method obtained a thick extract of komba-komba leaves. Komba-komba leaf extract ointment was made in three formulas based on differences in extract concentration: FI = 5%, FII = 10% and FIII = 15%). The ointment preparations obtained were subjected to organoleptic tests (color, odor, and consistency) and physical tests, including homogeneity, pH, viscosity, spreadability, stability tests, and scratch wound healing activity tests. The results showed that the extract’s concentration affected the ointment preparation's color, consistency, and physical properties. The effectiveness test results for healing scratches showed that the ointment preparation with an extract concentration of 15% provided an effective healing speed. Wed, 01 Jan 2025 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/10420 2025-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/10419 Title: The Characterization of Instant Granule Melinjo Peel (Gnetum gnemon Linn.) as Alternative Treatment for Hyperuricemia Authors: Senja Maelaningsih, Firdha; Permata Sari, Diah; Rahmah Fahriati, Andriyani; Raviola Saputra, Teungku; Al Ansori, Andika Abstract: Hyperuricemia is a non-communicable disease characterized by high levels of purine in the blood. One of the foods high in purine is melinjo chips. Instant granules from melinjo peel extract with a concentration of 450 mg have been proven to be effective as an anti-hyperuricemia treatment. To ensure the quality of the instant granule preparation, a quality evaluation was conducted to establish it as a new alternative in hyperuricemia therapy. The study aimed to assess the quality of the instant granule preparation of melinjo peel extract, including physical evaluation (particle size, loss on drying, and pH), characterization with Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FT-IR), and specific microbial tests. The test results revealed that the particle size of the instant granules was classified as coarse particles, the Loss on Drying results showed 1.29% granule loss, and the pH evaluation indicated that the granules had a pH of 7.78. Based on the SEM characterization, the granules exhibited non-clumping and heterogeneous morphology. FTIR analysis showed the presence of quercetin in the instant granules of melinjo peel. Specific microbial testing indicated the absence of E. Coli in the preparation. Therefore, it is concluded that the instant granule preparation of melinjo peel has characteristics that make it suitable for development as an instant nutraceutical granule preparation for anti-hyperuricemia treatment. Keywords: Gnetum gnemon peel; Hyperuricemia; Instant granule; Quality evaluation Wed, 01 Jan 2025 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/10419 2025-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/10418 Title: Formulation and Antioxidant Activity of Syrup Preparation Containing Saga Leaf Extract (Abrus precatorius L.) Authors: Sari Dewi, Bheta; Meitania Utami, Sheila; Sayyidah, Sayyidah; Season Alamsyah, Salwa; Tri Rahmadani, Novita Abstract: Saga leaf (Abrus precatorius L.) have tannins, saponins, alkaloids, and flavonoids that have functions as antioxidants. The plant belongs to the Fabaceae family and is widespread in the tropics and subtropics. Syrup is one form of pharmaceutical preparation used in providing drugs in liquid form so that it is easily drunk by patients, especially children. The purpose of this study was to obtain the best syrup preparation formulation physically and its antioxidant activity. Saga leaf are extracted in ethanol and then syrup preparations by adding saga leaf extract with concentration variations of 0.5% (FI), 1% (FII), and 2% (FIII). Evaluation of syrup preparations includes organoleptic, pH, homogeneity, and antioxidant activity test using DPPH (1,1-diphenyl-2-picrylhydrazyl) measured by UV-Vis spectrophotometer at wavelength 516 nm. The results showed that Formula III had the best results, namely clear dark brown organoleptic, slightly viscous shape, distinctive smell and slightly bitter taste, pH 6.15, homogeneous, and the preparation was stable in storage for 12 days and showed the best IC50 value of 116.94 g/mL compared to FI (132.65 g/mL) and FII (118.03 g/mL). The antioxidant activity of saga leaf extract is categorized as moderate. In conclusion, saga leaf can be formulated as a syrup preparation with an extract concentration of 2% in formula III showing effectiveness as the best IC50 value of 116.94 g/mL and the best formulation. Wed, 01 Jan 2025 00:00:00 GMT http://localhost:8080/xmlui/handle/123456789/10418 2025-01-01T00:00:00Z