http://localhost:8080/xmlui/handle/123456789/9073 2026-04-09T00:49:20Z http://localhost:8080/xmlui/handle/123456789/9214 Title: Interplay of host and viral factors in inflammatory pathway mediated cytokine storm during RNA virus infection Authors: Chaudhary, Riya; Meher, Aparna; Krishnamoorthy, Pandikannan; Kumar, Himanshu Abstract: RNA viruses always have been a serious concern for human health by causing several outbreaks, often pandemics. The excessive mortality and deaths associated with the outbreaks caused by these viruses were due to the excessive induction of pro-inflammatory cytokines leading to cytokine storm. Cytokines are important for cell-tocell communication to maintain cell homeostasis. Disturbances of this homeostasis can lead to intricate chain reactions resulting in a massive release of cytokines. This could lead to a severe self-reinforcement of several feedback processes, which could eventually cause systemic harm, multiple organ failure, or death. Multiple inflammation-associated pathways were involved in the cytokine production and its regulation. Different RNA viruses induce these pathways through the interplay with their viral factors and host proteins and miRNAs regulating these pathways. This review will discuss the interplay of host proteins and miRNAs that can play an important role in the regulation of cytokine storm and the possible therapeutic potential of these molecules for the treatment and the challenges associated with the clinical translation 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/9213 Title: Nanoparticles cellular uptake, trafficking, activation, toxicity and in vitro evaluation Authors: Toscano, Fernanda; Torres-Arias, Marbel Abstract: Nanoparticles (NPs) physicochemical properties, such as size, shape, surface chemistry, charge, etc., play a critical role in biological systems interactions, which include NPs’ cellular uptake, trafficking, activation, and toxicity. Although nano-bio interactions are multifaceted and complex, their assessment is essential for future therapeutic and diagnostic use since being carriers that deliver specific molecules (i.e., active pharmaceutical ingredients and imaging agents) in intracellular sites. The journey of NPs begins by reaching the plasma membrane and entering the cell mainly through endocytosis. After vesicles pinch off the cell membrane, the intracellular trafficking is mediated by a network of cellular endosomes which direct NPs to the different cellular components. Otherwise, NPs or their contents are released into the cytoplasm. In both cases, NPs can pass undetected or be recognized by the cell leading to a pro or anti-inflammatory response. Indeed, the cell response mostly depends on cell type and NPs physicochemical properties. The principal mechanism by which NPs activate the cell response is RONS production. Other mechanism includes signaling pathways modulation related to metabolic and enzymatic reactions, cell transduction, and immune modulation. Hence, the underlying mechanisms of cellular and subcellular interactions in vitro should be performed to provide insights into NPs’ effect. This information helps us to improve their synthesis and design to maximize the clinical benefits while minimizing side effects. Most in vitro tests to evaluate NPs’ effect in cells were developed focusing on cell dysfunctions, cytotoxicity, genotoxicity, immunogenicity, and cell death. 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/9211 Title: The efficacy, safety, and immunogenicity of plague vaccines: A systematic literature review Authors: Hartley, Louise; Harold, Sydney; Hawe, Emma Abstract: Plague remains endemic in many parts of the world, and despite efforts, no preventative vaccine is available. We performed a systemic review of available randomised controlled trials (RCTs) of live, attenuated, or killed plague vaccines vs. placebo, no intervention, or other plague vaccine to evaluate their efficacy, safety, and immunogenicity. Data sources included MEDLINE, Embase, and the Cochrane Library; clinical trial registers; and reference lists of included studies. Primary outcomes were efficacy, safety, and immunogenicity. Risk of bias was assessed using the Cochrane Collaborations tool. Only 2 RCTs, both on subunit vaccines, were included out of the 75 screened articles. The 2 trials included 240 participants with a follow-up of 3 months and 60 participants with a follow-up of 13 months, respectively. Safety evidence was limited, but both vaccines were well tolerated, with only mild to moderate adverse events. Both vaccines were immunogenic in a dose-dependent manner. However, given the limited data identified in this systematic review, we are unable to quantify the efficacy of vaccines to prevent plague, as well as their long-term safety and immunogenicity. More trials of plague vaccines are needed to generate additional evidence of their long-term effects. 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/9210 Title: Functional role of the TGF-β signaling in the Drosophila immune response Authors: Bastin, Ashley; Eleftherianos, Ioannis Abstract: TGF-β signaling pathways are present in diverse animal species, which indicates their evolutionary importance in modulating several conserved biological processes and maintaining host homeostasis by adjusting the activity of innate immune mechanisms. Drosophila melanogaster utilizes two related but separable cascades of the canonical TGF-β signaling pathway: The Bone Morphogenetic Protein and Activin branches. Recent studies have produced significant information on the immune role of TGF-β signaling in the fruit fly model during response against certain bacterial pathogens. Results from further investigations have generated novel insights into the role of Drosophila TGF-β signaling molecules as immune regulators opposing infection against nematode parasites and their mutualistic bacterial partners. This knowledge has revealed a previously unknown layer of the host innate immune system. Here we summarize these recent breakthroughs focusing on the participation of TGF-β signaling factors in various Drosophila immune processes in relation to infection with potent bacteria and nematode parasites. The presented information provides important clues indicating directions for future research into the design of novel strategies for the effective control of infectious diseases caused by bacterial pathogens and parasitic nematodes. 2023-01-01T00:00:00Z