http://localhost:8080/xmlui/handle/123456789/8371 2026-04-09T05:13:18Z http://localhost:8080/xmlui/handle/123456789/9635 Title: Optimization of Osteopontin Recombinant Protein as a Candidate Supplementation for Semen Preservation Authors: Hernawati, Tatik; Damayanti Lestari, Tita; Utama, Suzanita; Rimayanti, Rimayanti Abstract: The recombinant protein of heterologous proteins in Escherichia coli strains K12 has various and different systems tested and demands a detailed insight into the multiple factors affecting the encoded protein. One of the crucial factors is the acceptable quality of the DNA copies inserted inside the bacteria. Firstly, the amplification procedure needed to be performed well; thus, designing the primer and selecting the optimum annealing temperature are the focus indicators in this study. This study obtained a reference gene from the NCBI data bank with Reference Sequence: NM_174187.2. Two types of primers (SPP1FSPP1R and OPN1F - OPN1R) with different targeted bands were designed and selected after being reconstructed using the software. Online software such as addgene.org is also used to identify the right restriction site. The annealing temperature distinguished the PCR system used to amplify each primer. The result of this study revealed the best annealing at 65ºC successfully amplified 820 bp of the targeted band. The phenomenon not following the theory of blue-white screening is the empty plasmid control, where not a single colony grows on the media. Competent cells inserted with empty plasmids should still be able to expand on LB-Amp agar media because the presence of these plasmids is capable of providing resistance to antibiotics (in this case, ampicillin). This discrepancy is thought to have been caused by the improper insertion of the empty plasmid so that the plasmid did not enter the competent cell. Key words: Osteopontin, Primer, DNA clone, PCR amplification 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/9634 Title: Quantitative Analysist of Ethnomedicinal Practice and Used by the Banceuy Tribe in Subang Village of Indonesia Authors: Evalina Gondokesumo, Marisca; Qurrotu Aini, Silmi; Rahmadani, Siti Abstract: Background: The people of Banceuy, Indonesia have used plants for traditional medicine treatment for generation to generation. However, this local knowledge has not been recorded until today. The quantitative approach of ethnomedicinal documented show the usefulness of plant. More application medicinal plant, more pharmacological reported, more drug discovery potential development. This study aims to understanding the utilization of plants for medicinal treatment by the people of Banceuy tribe, Subang village, Indonesia. Methods: We conducted semi-structured interviews with a total of 35 informants that representative 10% of the total family units in Banceuy. The data has been analysed within species use value (SUV), family use value (FUV), plant part use (PPU), and the relative frequency of citation that was calculated based on fidelity level (FL). Result: We found 91 identified species and 3 unidentified species belonging of 41 group of families to treat 26 types of diseases. Among the recorded, Zingiberaceae and Piperaceae were the most abundant. The plant specieses with the highest SUV were Abelmoschus manihot (L.) Medik. (0.74) and Ageratum conyzoides L. (0.71). The leaves were found as the most used plant part and decoction was the dominant plants in medicinal administration. There were several unique traditional medicine treatments in Banceuy, such as tuak for cough ailment and post-partum treatment by mixed leaves concoction. Finally, all the data documented would be importance for new drug discovery, dried herbal market, as well as improving the society local income. Key words: Banceuy tribe, Traditional medicine treatment, Species and family use value, Plant part use 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/9633 Title: The Influence of Work Engagement on Job Satisfaction and Human Resource Performance in South Sulawesi Provincial Hospital 2022 Authors: Utami Husain, Nurfhadila; A Pasinringi, Syahrir; Rivai, Fridawaty Abstract: One of the most important factors for an individual to have at work is work engagement which is a concept that can reflect that an individual has passion, focus, and also strong dedication to work at the company. Work engagement is also defined as a positive and satisfying mental state related to work that is characterized by vigor, dedication, and absorption.1 An HR can be said to be work engagement when an HR is able to make his work a priority for himself and personally for the organization besides that work engagement is personal HR who are proud to have been part of an organization so that they are able to work according to the jobdesk given (Rohana Manalu et al., 2021). This study aims to analyze the effect of Work Engagement on Job Satisfaction and HR Performance at the South Sulawesi Provincial Hospital. This type of research is quantitative research using an analytic observational design with a cross sectional study approach. The sample in this study were health workers using a stratified random sampling technique of 316 people. The samples taken in this study were 152 employees at Laburan Baji Hospital, 122 Haji Hospital and 42 people at Sayang Rakyat Hospital. Data analysis used univariate, bivariate & path analysis. The results showed that there was a direct effect of work engagement on job satisfaction and HR performance with a coefficient of 0.525 and 0.267 for each with a p value (0.000) < 0.05 (significant). In addition, there is a direct effect of job satisfaction on HR performance, namely a coefficient value of 0.620 with a p value (0.000) <0.05. Meanwhile, the effect of work engagement on HR performance through job satisfaction obtained a coefficient value of 0.326 with a p value (0.000) < 0.05. which means Work Engagement has an influence on HR Performance through Job Satisfaction. Key words: Work engagement, Satisfaction, Performance. 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/9632 Title: Policy Implementation of Hypertension Prevention and Control Program in Banjarmasin Authors: Pobas, Sally; Nazaruddin, Balqis; Palutturi, Sukri Abstract: The Ministry of Health launched the Policy Implementation for the Prevention and Control of Hypertension Program with the goal of addressing the needs of national and regional public health development through minimal service standards. The goal of this study was to look at how hypertension prevention and control programs were implemented in the city of Banjarmasin. This study uses a qualitative descriptive-explorative analysis method with multiple case study approaches. Data collection was carried out by in-depth interviews, observation, documentation and literature study to all research informants. Informant selection technique used purposeful sampling. Research variables included communication, resources, bureaucratic structure and dispositions/attitudes. The research findings indicated that communication between policymakers, implementers, and program targets was effective. Despite the fact that human resources have reached the criterion for personnel, certain officers continue to have an excessive burden, and the number of health cadres was insufficient. Although facilities and infrastructure were available, several Public Health Centers were still having difficulty channeling demands and budgets. The bureaucratic structure had been implemented through SOPs and the delegation of authority, however numerous agencies still lacked complete documentation and archives. Even though the overall objectives were not attained, all parties exhibited a strong commitment and determination to continue the program. It can be concluded that the implementation of hypertension prevention and control policies in Banjarmasin had been quite successful, though there were still some challenges, such as differences in program target participation methods, the need for systematic equalization of authority tasks, and more equitable budget management in terms of resources. Furthermore, in terms of bureaucratic structure, it is vital to develop organized documentation standards in each agency. Key words: Implementation, Policy, Program, Prevention, Control, Hypertension. 2023-01-01T00:00:00Z