http://localhost:8080/xmlui/handle/123456789/8221 2026-04-09T06:43:25Z http://localhost:8080/xmlui/handle/123456789/8292 Title: The Effect of Temperature on Formaldehyde Migration and the Validation of Analytical Methods Used in Herbal Plastic Packaging Authors: Fatimah Nisma; Hurip Budi Riyanti; Delta Julanda Abstract: Plastic as food packaging has been the subject of extensive research, but plastic as packaging for herbs does not yet exist. As an additive, plastic made from the monomer and urea-formaldehyde is utilized. At elevated temperatures, formaldehyde can decompose due to the degradation of the monomer, followed by oxidation and the severing of the carbon chain. Formaldehyde can migrate to the bundled material, which is hazardous to human health. This study aimed to ascertain the impact of temperature on the migration of formaldehyde from plastic used to wrap herbs. The sample is a plastic bag used to wrap herbs, and NIR (Near Infrared) is used to determine the variety of plastic. Formaldehyde migration was determined by heating the sample between 40 and 80 degrees Celsius, and formaldehyde was analyzed using the UV-Vis spectrophotometer method with Nash reagent. The absorbance of formaldehyde was measured with a spectrophotometer at a wavelength of 412 nm. The type of plastic obtained was PP (Poly Propylene). The equation for the formaldehyde calibration curve is y = 0.0197x + 0.1218 with a correlation coefficient (r) of 0.999. The migration of formaldehyde was measured after heating plastic to temperatures between 40 and 80 degrees Celsius. The released formaldehyde concentrations ranged from 7.35 to 13.47 µg/mL. Validation of the analytical method revealed the formaldehyde detection limit (LOD) to be 0.8024 µg/mL and the quantity limit (LOQ) to be 2.6745 µg/mL, with a precision of 1 and an accuracy of 97,462-113,851%, thereby satisfying the meticulous, exhaustive, and precise criteria. 2023-08-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/8287 Title: Antioxidant and Inhibition Lipase Enzyme Activity of Centella asiatica Leaf Extract Authors: Yunarto, Nanang; Mawadatun, Ummi; Reswandaru, Uud Nourma; Sopian, Ahmad; Isnawati, Ani; Alegantina, Sukmayati Abstract: Hyperlipidemia and many other metabolic diseases are related to oxidative stress. Centella asiatica is a herbal medicine with a reported antioxidant effect in vitro. Centella asiatica contains secondary metabolites asiaticoside, which are included in the terpenoid group. The study evaluated the respective antioxidant potential and lipase enzyme inhibition of Centella asiatica leaves extract (CAE). Centella asiatica were extracted in ethanol, and the extract was assayed for the measurement of asiaticoside. Ethanolic extracts of asiaticoside content were prepared for HPLC analysis. The antioxidant potential of extracts was assessed by their free radical scavenging activity, such as 2, 2-diphenyl -1-picrylhydrazyl, as well as reducing. The anti-hyperlipidemic effect was evaluated in vitro lipase inhibitory activity test carried out enzymatically using the ELISA method with simvastatin as a comparison. The results showed asiaticoside contained in CAE 1.26%; the IC50 value of the antioxidant test of CAE was 11.38 g/mL; the IC50 value of the lipase enzyme in the CAE was 26.14 g/mL. The antioxidant activity of CAE is categorized as very strong and has the potential to inhibit lipase enzymes. The study suggests that CAE has the potential to inhibit lipase activity, suppressing lipid digestion and thereby diminishing the entry of lipids into the body 2023-08-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/8284 Title: Formulation of Serum Using a Combination of Tamanu Oil and Tea Tree Oil as Antiacne Authors: Ermawati, Dian; Cahyani, Pradina Zustrian; Shahnaz, Ifa; Juniarty, Amelya; Mahardhika, Chandra Leony; Chasanah, Uswatun Abstract: Acne is a chronic inflammation of the skin caused by bacteria. Tamanu oil contains neoflavonoids and tea tree oil contains terpenes-4-ol, both of which have good antibacterial agents. The combination of Tamanu oil and tea tree oil will produce a preparation synergistic in inhibiting bacterial growth. This study was to determine the best formula for serum preparations with good characteristics and stability against bacteria without irritating the skin. Serum formulation in three different concentrations of Tamanu oil is 0.5%, 0.75%, and 1%, each combined with 2% tea tree oil. Then the characteristics test (organoleptic, homogeneity, viscosity, dispersibility, and pH), stability test (organoleptic, homogenous, and pH) using cyclic temperature stress testing, and antibacterial test against Staphylococcus aureus and Propionibacterium acnes using well-diffusion method and irritation test using Hen’s egg test chorioallantoic membrane (HET-CAM) were carried out. The results showed that the overall viscosity, spreadability, and pH matched the specification, were stable, and had no irritation effect. Serum formulation containing Tamanu oil 1% and tea tree oil 2% is the best antibacterial activity which zones inhibition of 12.5 mm on Propionibacterium acnes and 11.6 mm on Staphylococcus aureus. 2023-08-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/8282 Title: Formulation of Instant Granules from Ethanolic Extract of Melinjo Peel (Gnetum gnemon L) Extract as Anti-Hyperuricemia Authors: Sari, Diah Permata; Fahriati, Andriyani Rahmah; Maelaningsih, Firdha Senja Abstract: Melinjo (Gnetum gnemon L) is extensively found in Indonesia, and all of its components are highly employed, with the fruit being the most commonly used. However, melinjo fruit can induce hyperuricemia if ingested excessively since it contains purines, which can raise uric acid levels. Several investigations have found that melinjo peel can lower uric acid levels in experimental rats in vivo. As a result, the goal of this study is to create and evaluate immediate granule formulations of melinjo peel extract. Plant determination, standardization of specified parameters, standardization of non-specific parameters, formulation, and physical and chemical assessment of instant granules of melinjo peel extract are all steps of the technique. This study employed two instant granule formulations of melinjo peel extract FI and FII with varying PVP concentrations (1g FI and 3g FII). Flow time testing, angle of repose testing, compressibility index testing, water content testing, and dissolving time testing were all part of the physical examination of instant granules. The chemical evaluation took the form of a UV-Vis spectrophotometer study of total flavonoid levels in instant extracts and granules. The FI and FII instant granule formulas had a yellow color, the flow time test results were in a good category (44 g/s and 81 g/s), the angle of repose test results was in the very good flow properties category (19° and 16°), the compressibility test results, the water content test, and the dissolving time test were performed in triple, in 0, 7th, and 14th day, and the results were in a good category. In conclusion, the physical features of instant granules of FI and FII melinjo peel extracts fulfilled the requirements for all test parameters and had a high possibility to be produced as anti-hyperuricemia preparations. 2023-08-01T00:00:00Z