http://localhost:8080/xmlui/handle/123456789/8220 1 - 44 2026-04-08T23:00:34Z http://localhost:8080/xmlui/handle/123456789/8230 Title: Unmeasurable HbA1c result due to hemoglobinopathy Authors: Aktas, Ayse; Ipek, Belkiz Ongen; Dikker, Okan Abstract: The aim of this study was to show the interference caused by hemoglobinopathy in the measurement of hemoglobin A1c (HbA1c). In our case presentation, we reported two patients whose HbA1c values were unmeasurable when using our laboratory's cation exchange chromatography. We detected HbSβ+ and HbSC variants by remeasuring the samples with another chromatography instrument. Measurement of HbA1c is a commonly performed procedure for the diagnosis of diabetes and for the assessment of blood glucose control in patients with diabetes. However, various hemoglobinopathies, chronic kidney disease, and abnormalities in red cell turnover rate may interfere with HbA1c quantification. 2024-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/8228 Title: Exploratory role of serum FGF-8 as a marker of bone metastasis in tumor progression Authors: Gill, Gurpreet Singh; Kaushik, Meenakshi; Kharb, Simmi; dkk. Abstract: Objectives: FGF-8, a member of the FGF family, plays a crucial role in cellular processes and has been implicated in cancer progression. The study aims to comprehend FGF-8's involvement in bone metastasis, emphasizing its potential as a diagnostic marker and focusing on its association with Bone-Alkaline Phosphatase (B-ALP) and other biochemical parameters. Methods: The case-control study spans 12 months, involving 60 participants, including 30 with secondary bone metastases and an equal number without metastasis. FGF-8 levels were quantified using ELISA, and B-ALP, serum ALP, and various biochemical parameters were assessed. The study employed standardized procedures to minimize bias, including matching cases and controls, and obtaining ethical approval. Results: In patients with bone metastasis, serum ALP levels, particularly B-ALP, were significantly higher. The metastatic group exhibited elevated FGF-8 concentrations, showcasing a positive correlation with B-ALP and serum calcium levels. The study successfully differentiated ALP isoenzymes through heat inactivation and L-phenylalanine inhibition. Additionally, serum calcium levels were markedly elevated in the metastatic group. Conclusion: The findings suggest that FGF-8 is a potential diagnostic marker for bone metastasis, particularly in breast and prostate cancers. Elevated FGF-8 levels correlate with increased B-ALP and serum calcium, indicating its role in osteoblastic differentiation in metastasis. The study proposes the utility of ELISA-based kits for FGF-8 in serum as a practical and efficient method for assessing bone tumor progression. 2024-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/8227 Title: Biomarkers of a Football Match-play - Internal load analysis using Technical Soccer Specific Aerobic Field Test (TSAFT90) Authors: Gopalakrishnan, Janani; Dharanirajan, Kaveen; Petra, Zanetta Brewart; dkk. Abstract: Objectives: The study measured the magnitude of physiological, immune, endocrine, and muscle damage markers of exercise-induced fatigue using the Technical Soccer Specific Aerobic Field Test (TSAFT90). We examined the effect of fatigue on performance and recovery at 24 hours post-exercise using biochemical indices. Methods: Professional football players (n=30) with a mean age of 19.20 years participated in the study during their preseason. To induce fatigue, participants underwent a 90-minute fatigue simulation program, TSAFT90. Venous blood samples were collected at baseline, 0-hour, and 24 hours post-fatigue. Analyzed markers included fatigue metabolites (lactate and uric acid), endocrine response marker (cortisol), muscle damage marker (creatine kinase), immunological markers (leukocytes, lymphocytes, neutrophils, and monocytes), inflammatory marker (CRP), hydration indicator (serum osmolality), and recovery marker (magnesium). Ball velocity and a 7-point Likert scale for muscle soreness were recorded to assess performance and perception of fatigue, respectively. Results: All biomarkers studied were significantly elevated (p<0.05) at 0 hours post-fatigue. Uric acid, creatine kinase, leukocytes, monocytes, CRP, serum osmolality, and magnesium remained altered at 24 hours. Ball velocity significantly reduced post-fatigue (p=0.04) from 94.67 km/hr to 90.47 km/hr, whereas there was no change in the soreness scale. Conclusion: The failure of the biomarkers to return to baseline levels within 24 hours indicates disrupted homeostasis. Monitoring the internal load with biomarkers aids in formulating strategies that can delay or mitigate fatigue and help achieve optimal performance and recovery, thus reducing the likelihood of injury. 2024-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/8225 Title: The association of serum proprotein convertase furin/PCSK3 concentrations with stable coronary artery disease and coroner artery lesion severity Authors: Yucesan, Fulya Balaban; Orem, Asim; Orem, Cihan; dkk. Abstract: Objectives: Furin (Proprotein Convertase Subtilisin/Kexin Type 3, PCSK3) is a proprotein convertase involved in the processing of precursor proteins. Furin substrates play significant roles in the initiation and progression of atherosclerosis, which is the primary cause of coronary artery disease (CAD). This study aimed to investigate the serum furin concentrations in stable CAD patients and their relationship with disease severity. Methods: The study included 81 stable CAD patients and 50 subjects without coronary artery lesions. Coronary angiography was performed via the percutaneous femoral artery approach, and the severity of CAD was assessed using the Gensini score. Serum furin concentrations were measured using an enzyme-linked immunosorbent assay. Results: Serum furin concentrations were significantly higher in CAD patients compared to CAD-negative subjects (p=0.0001). The serum furin levels of mild, moderate, and severe CAD patients were 1.53 ng/mL, 2.01 ng/mL, and 3.03 ng/mL, respectively, which were significantly different from CAD-negative subjects (p=0.018, p=0.002, and p=0.0001, respectively). Furin levels were found to be an independent predictor of CAD and exhibited potential diagnostic value for CAD and severe CAD. Conclusion: The study concluded that serum furin concentrations could be considered a new risk factor for CAD, in addition to well-known biomarkers. 2024-01-01T00:00:00Z