http://localhost:8080/xmlui/handle/123456789/7724 2026-04-14T20:40:00Z http://localhost:8080/xmlui/handle/123456789/7924 Title: Antibacterial activity of ethanol extracts of Hibiscus tiliaceus L. leaves from different extraction methods against Escherichia coli and Staphylococcus aureus Authors: Hidayati, Devi Nisa; Maghfiroh, Hanik Rohmatul; Safitri, Aysah Abstract: The leaves of sea hibiscus (Hibiscus tiliaceus L.) are known to have good antibacterial activity against Escherichia coli and Staphylococcus aureus, which is associated with the saponin, flavonoid, polyphenol, and tannin contents. Chemical compounds in plant extracts are, however, influenced by the extraction method used. This study aimed to determine the antibacterial activity of the ethanol extracts of Hibiscus tiliaceus L. leaves obtained using four different extraction methods against E. coli and S. aureus. In the maceration, percolation, reflux, or Soxhlet method, 200 g of the crude drug (i.e., dried sea hibiscus leaf powder) was extracted with 96% ethanol, and the derived extract was tested at 1%, 2%, 3%, 4%, and 5% concentrations. The disk diffusion method was used to test the antibacterial activity by observing and statistically analyzing the diameter of the zones of inhibition. Results showed that all the ethanol extracts have antibacterial properties against E. coli and S. aureus, with the maceration-produced extract forming significantly different zones of inhibition from the other methods (percolation, reflux, and the Soxhlet) at all concentrations. 2023-03-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7923 Title: Antioxidant compounds and activity from the leaf of the mistletoe (Dendrophthoe pentandra (L.) Miq.) on Duku plant (Lansium domesticum Corr.) Authors: Salni; Marisa, Hanifa; Mayasari, Putri Abstract: Mistletoe, known in Indonesia as benalu, is a parasitic plant that can thrive on a variety of hosts. This study determined that Dendrophthoe pentaphylla (L.) Miq. was accountable for the antioxidant activity of mistletoes growing on Duku (Lansium domesticum Corr.). This study aims to assess the antioxidant potency in the fractions of n-hexane, ethyl acetate, and methanol-water, as well as to identify the chemical groups and IC50 values. Extraction by maceration, fractionation by liquid fractionation method, isolation by vacuum liquid chromatography (VLC) and column chromatography, and DPPH assay for antioxidant activity were employed in this study. The n-hexane and ethyl acetate fractions exhibited antioxidant activity, but the methanol fraction showed minuscule activity. NH.1 (compounds from the N-hexane fraction followed by VLC were found in eluate 1) and NH.3 (compounds from the N-hexane fraction followed by VLC were found in eluate 3) were successfully isolated from the n-hexane fraction. The former was identified as terpenoids, while the latter belonged to flavonoids. The ethyl acetate fraction yielded the flavonoid EA.3 (compounds from the ethyl acetate fraction followed by VLC were found in eluate 3), which was isolated from the ethyl acetate fraction. Compound NH.1 showed weak antioxidant activity indicated by IC50 value of 151.35 g/mL. Meanwhile, NH.3 and EA.3 were categorized as moderate, proven by IC50 values consecutively of 96.64 and 78.37 g/mL. 2023-03-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7921 Title: The effect of temperature solubility on particle size and antibacterial activity of nanosilver of gembili’s inulin Authors: Ermawati, Dian Eka; Fajrin, Hayunda I’zaaz; K, M. Nur Dewi; Rohmani, Sholichah Abstract: A local Indonesian tuber such gembili (Dioscorea esculenta L.) is reported as a source of inulin potentialy. Inulin is one of the fiber types and carbohydrate sources derived from plants and has prebiotic and antibacterial activity. However, inulin long chain has low solubility in water, so it is necessary to modify the temperature solubility in the biosynthetic process to enhance its solubility. In this study, the biosynthetic process used inulin as a reducer of silver ions. And also to help increase the absorption of inulin. Gembili inulin was mixed with silver nitrate solution at variation temperatures of 25 ℃, 60 ℃, and 90 ℃ of a biosynthetic process to produce a nano particle size and antibacterial activity against Staphylococcus aureus and Escherichia coli bacterias. This study showed that nanosilver biosynthetic has a a particle size of 12-49 nm with a round shape. The temperature solubility of inulin affect to particle size, but did not show any significant difference toward the antibacterial activity against S.aureus and E.coli bacterias. FTIR spectra showed an interaction between silver nitrate and inulin of gembili functional groups. Inulin acts as a capping agent of nanosilver but does not induce the antibacterial activity. 2023-03-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7919 Title: Formulation and evaluation of orally disintegrating film (ODF) containing piperine-succinic acid cocrystal Authors: Hasanah, Uswatul; Fitriani, Lili; Putri, Verlia Nisrina; Zaini, Erizal Abstract: Piperine is a type of alkaloid found in plants of the Piperaceae family, including Piper nigrum and Piper longum, which has numerous pharmacological properties and is being developed as a nutraceutical. However, its poor solubility makes delivering in effective doses for therapeutic purposes challenging. To overcome this limitation, the cocrystals of piperine and succinic acid was prepared, which are more soluble, and then formulated into orally disintegrating films (ODFs) for better delivery. The cocrystals were formed using the slurry method, while the ODFs were made using the solventcasting method with different concentrations of hydroxypropyl methylcellulose (HPMC) and polyethylene glycol (PEG) 400. The resulting films were evaluated for various parameters, such as their organoleptic characteristics, weight and thickness uniformity, pH, moisture content, swelling properties, and disintegration time. The results showed that the ODF with 6% HPMC as a film-forming polymer and 0.6% PEG 400 as a plasticizer loaded with 10 mg of piperine in the form of piperine-succinic acid cocrystal was the best formula. This ODF formulation disintegrated in less than one minute and was found to be a good film with uniform content. 2023-03-01T00:00:00Z