http://localhost:8080/xmlui/handle/123456789/7456 207 - 426 2026-04-08T23:06:40Z http://localhost:8080/xmlui/handle/123456789/7509 Title: Effect of Allium sativum and Olea europaea on serum lipids in patients with diabetes mellitus Authors: Memon, Ali Raza; Rajput, Muhammad Ali; Rizwan, Fazeela; dkk. Abstract: Objective: This study was designed to assess the effects of a combination of Allium sativum and Olea europaea oil on disturbed lipid profiles in patients with type 2 diabetes mellitus (T2DM). Methods: This randomized control trial (RCT) involved 160 patients of either sex (aged 40e60 years) with T2DM and dyslipidemia, and were equally divided into two groups. Group A patients received hypoglycemic and lipid lowering agents (Tab glimepiride 2 mg þ metformin HCl 500 mg and Tab rosuvastatin 10 mg once a day orally). Patients in group B were given the same allopathic drugs as group A, in combination with A. sativum and O. europaea oil over a period of 6 months. Blood samples were taken at three stages of the study to allow the analysis of lipid profiles. Results: Analysis showed that after 3 and 6 months of treatment, the mean levels of serum cholesterol, triglycerides (TGs) and low-density lipoprotein (LDL) were reduced in both groups and that there was a highly significant (P < 0.001) decline in group B when compared to group A. High-density lipoprotein (HDL) levels increased in both groups but there was a significant (P < 0.05) increase in the group B when compared to group A. The percentage reduction was highest at 6 months of treatment for cholesterol, TGs, LDL and HDL in group B (41.5%, 45.9%, 60.4% and 58.1%, respectively). Conclusion: The antihyperlipidemic activity observed may be due to the presence of antioxidants in the test substances. Further studies should be conducted with a larger sample size in order to further evaluate the role of A. sativum powder and O. europaea oil in patients with T2DM with dyslipidemia. 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7507 Title: Clinical and laboratory presentation of von Willebrand disease: Experience from a single center in Saudi Arabia Authors: Owaidah, Tarek; Alharbi, Muhammed; Mandourah, Mohammed; dkk. Abstract: Objectives: This study was aimed at assessing the clinical presentations and laboratory findings among patients diagnosed with vWD at a Saudi tertiary care unit. Methods: This retrospective study included 189 patients with vWD who were followed up in our unit over 4 years. Clinical and laboratory data were collected and analyzed in SPSS. Results: The median age of the study cohort was 30 years (range 11 monthse56 years). The cohort had a female preponderance, with 32.30% males and 66.70% females. Bleeding from different sites was observed, mostly from the joints and muscles (23.90%), followed by the mucus membranes (14.60%), genitourinary areas (7.70%), ecchymoses (2.80%), and gastrointestinal areas (2.80%). A total of 48% of participants presented with more than one type of bleeding. A total of 105 (58.01%) participants had type 1; 29 (16.02%) had type 2; and 47 (25.96%) had type 3 vWD. Blood tests indicated the following mean value: hemoglobin, 116 25.60 gm/L; ferritin, 75.80 166.80 mg/L (median 28.5); vWAg, 0.40 0.27IU/ml; and vWD:RCo, 0.32 0.20IU/dL. The partial thromboplastin time was prolonged in 49.20% and normal in 50.80% of participants. Platelet function analysis values were prolonged in 92.90% and normal in 7.10% of participants. Comparative analysis of the O-type and non-O blood type showed that blood type O was significantly correlated with factor VIII (pvalue ¼ 0.013), vWF:RCo (p-value ¼ 0.004), and vWF:Ag (p-value ¼ 0.019). Conclusion: Joint and muscle bleeds were the most common clinical presentations in our cohort. Although type 1 vWD was most prevalent in our cohort, we observed a comparatively higher prevalence of type 3, possibly because of ethnic differences or referral bias. We found a significant difference between O and non-O blood type regarding FVIII and vWF:Ag, and observed a more pronounced difference for vWD activity measuresd by vWF:RCo with blood type O being the systematic factor. 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7505 Title: Novel biosynthesis of tellurium nanoparticles and investigation of their activity against common pathogenic bacteria Authors: Abed, Nermine N.; El-Enain, Inas M.M. Abou; Helal, Eman El-Husseiny; Yosri, Mohammed Abstract: Objectives: Tellurium has received substantial attention for its remarkable properties. This study performed in vitro and in vivo testing of the antibacterial action of tellurium nanoparticles biosynthesized in actinomycetes against methicillin-resistant Staphylococcus aureus (MRSA), a common blood bacterial pathogen. Methods: Nine actinomycete isolates were tested for their potential to reduce potassium tellurite (K2TeO3) and form tellurium nanoparticles (TeNPs). The most efficient actinomycete isolate in producing Tellerium nanoparticles was identified through molecular protocols. The generated TeNPs were characterized using UV, TEM, EDX, XRD and FTIR. The bacterial species implicated in bloodstream infections were detected at El Hussein Hospital. Bacterial identification and antibiotic susceptibility testing were performed using Vitek 2. An animal infection model was used to test the efficacy of the produced TeNPs against the most commonly isolated methicillin-resistant S. aureus using survival assays, colony counting, cytokine assessment and biochemical testing. Results: The most efficient actinomycete isolate was identified as Streptomyces graminisoli and given the accession number (OL773539). The mean particle size of the produced TeNPs was 21.4 nm, and rods and rosette forms were observed. Methicillin-resistant S. aureus (MRSA) was the main bacterium (60%) causing blood stream infections, and was followed by Escherichia coli (25%) and Klebsiella pneumoniae (15%). The produced TeNPs were tested against MRSA, the bacterium most frequently isolated from blood, and showed a promising action inhibition zone of 24 0.7 mm and an MIC of 50 mg/ml. An animal infection model indicated the promise of TeNPs alone or in combination with standard drugs to combat MRSA in a rat intravenous infection model. Conclusion: TeNPs combined with vancomycin have successive impact to combat bacteremia for further verification of results. 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7504 Title: Histomorphometry of the cortical layers and the dentate nucleus of the human fetal cerebellum Authors: Poudel, Phanindra P.; Bhattarai, Chacchu; Ghosh, Arnab; Kalthur, Sneha G. Abstract: Objectives: This study was aimed at determining the histomorphometry of the cerebellar cortical laminae and the dentate nucleus of the human fetal cerebellum; the number and shape of the neurons; and the gestational age of appearance of the cerebellar folia, white matter and arbor vitae cerebelli. Methods: Microscopic sections of the human fetal cerebellum stained with hematoxylin and eosin and Bielschowsky silver stain were studied. Results: The thickness of the cortical laminae of the human fetal cerebellum varied among gestational weeks as follows: external granular layer: 36.06 9.36 e50.05 34.06 mm, molecular layer: 32.76 17.16 e52 28.6 mm, Purkinje cell layer: 9.36 6.8 e15.6 4.68 mmand internal granular layer: 66.65 24.42 e146.63 47.79 mm. Similarly, the number of neurons per field of view at 1000X under a compound microscope varied among gestational weeks as follows: external granular layer: 89.92 42e142.84 50, molecular layer: 15 12.5e25 8.25, Purkinje cell layer: 3.5 1e5 2.5 and internal granular layer: 98.5 69.75e224 47. White matter in the fetal cerebellum was already present at the age of 12th gestational week, whereas cerebellar folia appeared at 16e20 gestational weeks. Arbor vitae cerebelli and the dentate nucleus became conspicuous after the 20th gestational week. Fetal neurons were round except for Purkinje cells. Conclusions: The thickness and neuronal counts of the human fetal cerebellar cortical layers and the measurements of the dentate nucleus along with other histomorphological features varied with gestational age from the 12th week of gestation until birth. 2023-01-01T00:00:00Z