http://localhost:8080/xmlui/handle/123456789/6721 2026-04-09T05:13:17Z http://localhost:8080/xmlui/handle/123456789/6824 Title: Mefenamic acid inhibit transforming growth factor-beta type-1: Repurposing anti-inflammatory drugs in wound healing using in-silico approaches Authors: Roney, Miah; Rashid Issahaku, Abdul; Binti Zamri, Normaiza; Fadhlizil Fasihi Mohd Aluwiv, Mohd Abstract: Mefenamic acid inhibit transforming growth factor-beta type-1: Repurposing anti-inflammatory drugs in wound healing using in-silico approaches Miah Roney a,b, Abdul Rashid Issahaku c,d, Normaiza Binti Zamri a, Mohd Fadhlizil Fasihi Mohd Aluwi a,b,* a Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang Darul Makmur, Malaysia b Centre for Bio-Aromatic Research, Universiti Malaysia Pahang Al-Sultan Abdulah, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang Darul Makmur, Malaysia c Department of Chemistry, University of the Free State, 205 Nelson Mandela Avenue, Bloemfontein 9301, South Africa d West African Centre for Computational Research and Innovation, Ghana A R T I C L E I N F O Handling Editor: Prof A Angelo Azzi Keywords: Mefenamic acid In-silico Wound healing Docking MD simulation A B S T R A C T Due to low cost and time-saving benefits, drug repurposing is a safe and successful method to discover drug. A druggable target for inflammation, transforming growth factor-beta type-1 (TGF-β 1) has been identified to be associated with wound healing. Finding the most effective TGF-β 1 inhibitor among FDA-approved anti-inflammatory medications was the goal of the current investigation. To find the best hits against TGF-β 1, we used structure-based virtual screening on medications that have received FDA approval. We discovered two FDAapproved medications with notable selectivity and affinity for the binding pocket of TGF-β 1. Mefenamic acid, one of these found hits, interacts with key TGF-β 1 residues and favourably attaches to the binding pocket, requiring further study. The kinetics of the binding between mefenamic acid and TGF-β 1 were revealed by allatom precise molecular dynamics (MD) simulations. Mefenamic acid, which may also be used as a possible lead chemical against TGF-β 1, may be a promising TGF-β 1 inhibitor. 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/6814 Title: Academic examination stress: Effects on salivary cortisol, neuropeptide Y and interleukin-1β Authors: S¸emsi, Rabia; Ergünol, Erdal; Kanad Er, Emre; Sepici Dinçel, Aylin Abstract: Academic examination stress: Effects on salivary cortisol, neuropeptide Y and interleukin-1β Rabia S¸emsi a,*, Erdal Ergünol b,c, Emre Kanad Er d,e, Aylin Sepici Dinçel a a Department of Medical Biochemistry, Faculty of Medicine, Gazi University, Ankara, Türkiye b Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Cyprus Health and Social Sciences University, Morphou, Cyprus c Alter Group, Innovation, Education, Consultation and Organisation Company, ˙Istanbul, Türkiye d Department of Prosthodontics, Faculty of Dentistry, Cyprus Health and Social Sciences University, Morphou, Cyprus e Department of Prosthodontics, Faculty of Dentistry, Cyprus Internatıonal University, Nicosia, Cyprus A R T I C L E I N F O Handling Editor: Prof A Angelo Azzi Keywords: Academic stress Saliva Neuropeptide Y Interleukin-1β A B S T R A C T Saliva is one of the preferred non-invasive body fluids for biomarker studies. This study aimed to investigate the possible alteration of stress biomarkers of the students before and after the examinations via salivary cortisol, neuropeptide Y (NPY), and Interleukin-1β (IL-1β) levels. Forty-four adults were included in the study and divided into groups of pre-examination (Group I) and post-examination (Group II). Salivary samples were collected between 8 and 9 a.m. before and after the exam, which ended at 5 p.m. by SARSTEDT saliva collection tubes. Participants were asked to soak the swab with saliva and take it out after 1 min. Swabs were kept at room temperature for 15–30 min and centrifuged for 10–15 min at 1500 g. Salivary cortisol (ng/mL), NPY (ng/mL), and IL-1β (pg/mL) levels were analyzed by Enzyme-linked immunosorbent assay (ELISA). The salivary cortisol, NPY and IL-1 β levels were significantly increased in Group II compared to Group I (9.65 ± 4.53, 6.37 ± 4.14, p < 0.019; 32.12 ± 4.69, 27.10 ± 4.71 p < 0.001; 11.69 ± 3.61, 7.20 ± 3.49, p < 0.0003 respectively). The IL-1β levels were positively and significantly correlated with salivary cortisol and NPY levels in Group II (r = 0.642, p = 0.03; r = 0.589, p = 0.004, respectively). Also, IL-1β levels were positively and significantly correlated with salivary NPY levels in Group I (r = 0.430, p = 0.04). These data indicated that acute stress can alter the inflammatory response and increase NPY release, which is positively associated with cortisol. 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/6806 Title: Gut microbiome associated dysbiosis: Limited regimens and expanding horizons of phage therapy Authors: Singha, Biplab; Singh Rawat, Bhupendra; Venkataraman, Ramya; Nair, Tripti Abstract: Gut microbiome associated dysbiosis: Limited regimens and expanding horizons of phage therapy Biplab Singha a, Bhupendra Singh Rawat b, Ramya Venkataraman c, Tripti Nair d, Eric H. Rosenn e, Vijay Soni f,* a Department of Microbiology and Physiology Systems, University of Massachusetts Chan Medical School, Worcester, MA, 01605, USA b Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Newark, NJ, 07103, USA c Laboratory of Innate Immunity, National Institute of Immunology, New Delhi, 110067, India d Leonard Davis School of Gerontology, University of Southern California, Los Angeles, USA e Department of Biomedical Engineering, Boston University School of Engineering, Boston, MA, 02215, USA f Division of Infectious Diseases, Weill Department of Medicine, Weill Cornell Medicine, New York, NY, 10065, USA A R T I C L E I N F O Keywords: Bacteriophage Phage therapy Gut microbiome Antimicrobial resistance Precision medicine Microbial dysbiosis A B S T R A C T Human gut microbiota plays an important role in health, broadly influencing metabolism to the immune system and drug resistance to pathogenic colonization. Since antibiotic resistance is on the rise, and wide-spectrum antibiotics are known to have deleterious effects on microbial biodiversity targeted therapeutic interventions must be made. Bacteriophages are viruses that are commonly recognized to have a high level of specificity, targeting only the intended bacterial species without disrupting the overall microbial community. Advancements in genomics, bioinformatics, and synthetic biology led us to the identification and design of phages, capable of precisely targeting specific pathogens. In this review article, we aim to discuss both the challenges and opportunities of integrating phage therapies into clinical practice, discussing the limitations of traditional therapy as it pertains to the manipulation of the gut microbiome. 2023-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/6805 Title: Inhibitory effect of Sinapic acid derivatives targeting structural and non-structural proteins of dengue virus serotype 2: An in-silico assessment Authors: Roney, Miah; Dubey, Amit; Binti Zamri, Normaiza; Fadhlizil Fasihi Mohd Aluwi, Mohd Abstract: Inhibitory effect of Sinapic acid derivatives targeting structural and non-structural proteins of dengue virus serotype 2: An in-silico assessment Miah Roney a,b, Amit Dubey c,d, Normaiza Binti Zamri a, Mohd Fadhlizil Fasihi Mohd Aluwi a,b,* a Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang Darul Makmur, Malaysia b Centre for Bio-Aromatic Research, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang Darul Makmur, Malaysia c Department of Pharmacology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, 600077, Tamil Nadu, India d Department of Computational Chemistry and Drug Discovery Division, Quanta Calculus, Greater Noida, 201310, Uttar Pradesh, India A R T I C L E I N F O Handling Editor: Prof A Angelo Azzi Keywords: Anti-dengue Sinapic acid In-silico Docking Pharmacokinetics A B S T R A C T DENV infects 50–100 million individuals, and 500,000 of them go on to acquire the more serious dengue hemorrhagic fever, which causes around 20,000 fatalities every year. Despite its widespread nature, there is no medication licenced to treat this condition. The purpose of this work is to identify anti-DENV medicines from sinapic acid (SA) derivatives utilising in-silico evaluation through docking and pharmacokinetics investigations. For the DENV-2 envelop protein, 1-O-β-D-glucopyranosyl sinapate had a significant docking score of 􀀀 7.7 kcal/ mol, while sinapoyl malate had a docking score of 􀀀 6.7 kcal/mol for the DENV-2 NS2B/NS3 protein. Additionally, according to the PASS server, 1-O-β-D-glucopyranosyl sinapate and sinapoyl malate have a wide range of enzymatic activities since their probability active (Pa) values is > 0.700. These compounds exhibit a numerous pharmacological effect through activating the body’s enzymes, according to analyses of their pharmacokinetic qualities. Accordingly, these substances showed acute toxicity rates at LD50 log10 (mmol/g) and LD50 (mg/g) concentrations when administered via various routes, including intraperitoneal, intravenous, oral, and subcutaneous. The result of this research suggests, 1-O-β-D-glucopyranosyl sinapate and sinapoyl malate may function as possible inhibitors to halt the DENV, and more in-vitro and in-vivo research is required to validate their activity and other features. 2023-01-01T00:00:00Z