http://localhost:8080/xmlui/handle/123456789/4837 2026-04-15T18:22:43Z http://localhost:8080/xmlui/handle/123456789/4966 Title: Potential Antihyperlipidemia Effect of Lactoferrin in Hyperlipidemia-Induced Male Sprague–Dawley Rats Authors: Fabio Jonathan Jusni1, Louis; Chandra, Valencia; Djuartina, Tena; Notario, Dion; Arieselia, Zita; Hananta, Linawati Abstract: Potential Antihyperlipidemia Effect of Lactoferrin in Hyperlipidemia-Induced Male Sprague–Dawley Rats Louis Fabio Jonathan Jusni1, Valencia Chandra1, Tena Djuartina2,3, Dion Notario4, Zita Arieselia5, Linawati Hananta5* 1Medicine Study Programme, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia 2Department of Anatomy, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia 3Biomedical Sciences Programme, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia 4Department of Pharmacy, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia 5Department of Pharmacology, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia Abstract Background: Hyperlipidemia is a condition that is characterized as an increase in total cholesterol and triglyceride levels in the blood. Lactoferrin is a protein that can serve as an antioxidant. This study aims to determine whether lactoferrin can reduce total cholesterol and triglyceride levels. Methods: This study used 24 Sprague–Dawley rat strains, which were divided into six groups: normal group; negative control; positive control; and dose groups 1, 2, and 3. The normal group was given standard feed, whereas the other group was given high cholesterol and fat. The positive control group and dose groups 1, 2, and 3 were given 1.5 mg/kg BW of simvastatin and 100, 200, and 400 mg/kg of BW lactoferrin, respectively. After 6 weeks, total cholesterol and triglyceride levels were measured. Results: This study showed that lactoferrin doses of 100, 200, and 400 mg/kg BW could significantly reduce total cholesterol and triglyceride levels (p < 0.05). Lactoferrin could also significantly reduce activated Kupffer cell and steatosis area in the liver (p < 0.05). Conclusions: Lactoferrin can reduce total cholesterol and triglyceride levels. Thus, further research is needed to address the existing bias and confirm that lactoferrin can reduce cholesterol and triglyceride levels. Keywords: cholesterol, hyperlipidemia, lactoferrin, sprague–Dawley, triglyceride 2022-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/4965 Title: Apoptotic Effect of Bortezomib on Pancreatic Islet Cells in STZ-induced Diabetic Rats Authors: Ekin, Çiğdem; Tekin Karacaer, Neslihan; Tarhan Karaoğlan, Mehtap; Örün, İbrahim; Öztürk, Kamile Abstract: Apoptotic Effect of Bortezomib on Pancreatic Islet Cells in STZ-induced Diabetic Rats Çiğdem Ekin1,2, Neslihan Tekin Karacaer3, Mehtap Tarhan Karaoğlan1, İbrahim Örün1, Kamile Öztürk1* 1Molecular Biology Division, Biology Department, Science and Letter Faculty, Aksaray University, Aksaray 68100, Turkey 2Adacell Translational Research Center, Dışkapı Yıldırım Beyazıt Traning and Research Hospital, Ankara 06145, Turkey 3Department of Molecular Biology and Genetics, Science and Letter Faculty, Aksaray University, Aksaray 68100, Turkey Abstract Background: This study aimed to investigate the possible apoptotic role of bortezomib (BMZ) on pancreatic islets of streptozotocin (STZ)-induced diabetic rats. Methods: Sprague-Dawley rats were divided into groups that were administered BMZ alone or in combination with STZ. To evaluate the effect of BMZ on the development of diabetes, blood glucose levels were measured regularly in the animals. Islet cell viability was determined by staining the islets with fluorescein diacetate and propidium iodide. Expression of the Bcl-2 and bax genes was determined in islet cells by quantitative real-time polymerase chain reaction. Results: Administering STZ-induced hyperglycemia in the rats reduced the viability of islet cells and the bcl-2/bax ratio. In the group administered BMZ alone, the bcl-2/bax gene expression rate in islets increased significantly compared to the control group. BMZ co-administered with STZ significantly increased islet cell viability and the bcl-2/bax ratio compared to the diabetic group. Conclusions: This study demonstrates that BMZ may protect pancreatic islet cells from apoptosis by increasing islet viability and upregulating the bcl-2/bax gene expression ratio, even though it failed to protect against the destructive effect of STZ. Keywords: apoptosis, bortezomib, pancreatic islets, rats, type 1 diabetes 2022-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/4956 Title: Ratio of Vascular Pedicle Width and Thoracic Diameter to Differentiate Cardiogenic and Non-Cardiogenic Pulmonary Edema Authors: Afifi, Rahmi; Fachri, Achmad; Sjarifuddin Madjid, Amir; Prihartono, Joedo; Prasetyo, Marcel; Christian, Andreas Abstract: Ratio of Vascular Pedicle Width and Thoracic Diameter to Differentiate Cardiogenic and Non-Cardiogenic Pulmonary Edema Rahmi Afifi1*, Achmad Fachri1, Amir Sjarifuddin Madjid2, Joedo Prihartono3, Marcel Prasetyo1, Andreas Christian1 1Department of Radiology, Faculty of Medicine, Universitas Indonesia–Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta 10430, Indonesia 2Department of Anaesthesiology and Intensive Care, Faculty of Medicine, Universitas Indonesia–Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta 10430, Indonesia 3Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia Abstract Background: Excess intravascular volume evaluation is essential in the intensive care unit (ICU); however, clinical information to differentiate cardiogenic and non-cardiogenic pulmonary edema has been proven ineffective. Thus, this study aimed to distinguish cardiogenic from non-cardiogenic pulmonary edema using the ratio of vascular pedicle width (VPW) to thoracic diameter (VPTR). Methods: This cross-sectional study was conducted based on secondary data from chest radiographs of 100 patients with clinical symptoms of pulmonary edema in the ICU from January 2013 to December 2015. Cardiogenic and non-cardiogenic pulmonary edema were distinguished using VPW and cardiothoracic ratio measurements (CTR). VPTR was measured to differentiate between the two types of pulmonary edema, and the cut-off value was obtained using a receiver operating characteristic curve. Results: This study revealed a prevalence of 21% and 79% for cardiogenic and non-cardiogenic pulmonary edema, respectively. A VPTR cut-off value of 25.1% with a sensitivity of 90% and specificity of 86%, may distinguish cardiogenic from non-cardiogenic pulmonary edema. Conclusions: VPTR is an alternative method to differentiate between cardiogenic and non-cardiogenic pulmonary edema, and this ratio measurement is useful in cases where radiograph films are not standardized. Keywords: blood vessels, critical illness, diagnostic imaging, intensive care units, pulmonary edema, radiography 2022-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/4951 Title: Association of Smoking with Total Oxidant and Antioxidant Levels in Breast Milk Authors: Yildiz, Nesibe; Yilmaz, Adnan Abstract: Association of Smoking with Total Oxidant and Antioxidant Levels in Breast Milk Nesibe Yildiz1*, Adnan Yilmaz2 1Department of Healthy Care Services, Vocational School of Health Services, Artvin Coruh University, Artvin 08000, Turkey 2Department of Medical Biochemistry, Faculty of Medicine, Recep Tayyip Erdogan University, Rize 53100, Turkey Abstract Background: Breast milk is a natural food that contains all the fluids, energy, and nutrients necessary for the optimum growth and development of newborns. Smoking is a public health problem that has harmful effects on the mother and baby. This study aimed to examine the association of exposure to smoking with total oxidant status (TOS) and total antioxidant status (TAS) in breast milk. Methods: Healthy mothers without any health problems during their pregnancy and lactation periods were selected as subjects. Eighty-eight milk samples (44 in the smoking group and 44 in the nonsmoking group) were examined. TOS and TAS were analyzed using Rel Assay commercial kits. Results: TAS level was significantly lower in the smoking group than in the nonsmoking group (p < 0.05). TOS level was higher in the smoking group than in the nonsmoking group, although the difference was not statistically significant (p > 0.05). Oxidative stress index (OSI) was significantly higher in the smoking group than in the nonsmoking group (p < 0.05). Conclusions: Exposure to smoking was associated with low TAS and high OSI in breast milk. Keywords: cigarette smoke, human breast milk, oxidative stress, total antioxidant level, total oxidant level 2022-01-01T00:00:00Z