http://localhost:8080/xmlui/handle/123456789/8013 2026-04-09T00:48:20Z 2026-04-09T00:48:20Z Sukmana, Judya Widjiati, Widjiati Siswandono, Siswandono http://localhost:8080/xmlui/handle/123456789/9572 2025-01-07T08:13:33Z 2022-01-01T00:00:00Z

Title: Leaf Effect of C. Trifolia L. as Nf-B and Tnf-Α Inhibitor Compounds with In Silico Method Authors: Sukmana, Judya; Widjiati, Widjiati; Siswandono, Siswandono Abstract: Introduction: Infection H. pylori causes inflammation through various pathways to induce proinflammatory cytokines such as IL-1, IL-6, IL-8, and TNF-α. The transcription factor NF-kB is a crucial regulator of the immune response and inflammation and regulates many cellular processes that are important in carcinogenesis, including transformation, proliferation, angiogenesis, and metastasis. Antiinflammatory plant C. trifolia L was shown to inhibit the activity of NF-B and several pro-inflammatory cytokine mediators. This study proved that the active compound from the plant's leaves, C. trifolia L has potential as an inhibitor of NF-B and TNF-α. Method: This study used a docking method with a grid box mimicking the bond between the receptor and the inhibitor control complex. Results: The bioactivity of Cayratria trifolia compounds as anti-inflammatory was shown in the inflammation parameters used, namely Interleukin 10 agonist, Interleukin agonist, Interleukin antagonist, Interleukin 6 antagonist, Interleukin 4 antagonist, Interleukin 2 agonist, Interleukin 1 antagonist, Interleukin 1b antagonist, Interleukin 10 antagonist, Interleukin 12 agonist, and Interleukin 1a antagonist. Interleukin 2 agonists showed the highest activity of all compounds. Piceid compounds showed high anti-inflammatory activity with interleukin 10 agonists, interleukin agonists, interleukin 6 antagonists, and interleukin 2 agonists. The compounds stilbenes, piceid, resveratrol, cyclopentadecane, and hentriacontane showed potency higher interleukin-6 inhibition than the other 22 compounds. These five compounds were continued for molecular docking analysis. The low bond energy is correlated with the number of bonds and the variety of interactions. The higher the number of bonds and the type of interaction, the lower the bond energy. The lower the bond energy, the stronger the interaction between the ligand and protein. Conclusion: Based on the prediction of anti-inflammatory bioactivity, five potential compounds were identified, namely cyclopentadecane, resveratrol, stilbenes, piceid, and hentriacontane. The five compounds bind to NFkB on the active site of the binding site with DNA, and this inhibition causes DNA to be unable to restrain NFkB transcription factors, and transcription does not occur. This proves that the active compound from the leaves of the plant C. trifolia L has potential as an inhibitor of NF-κB compounds. Inhibition of 6 compounds on TNF at the TNF receptor proves that the active compound from the leaves of the plant C. trifolia L has potential as a TNF-α inhibitor compound. The active ingredient Piceid exhibits predominant anti-inflammatory potential with lower binding energy and stronger interactions than other complexes. Key words: H. Pylori, NFkB, TNF-α, C. trifolia L, In silico

2022-01-01T00:00:00Z Sofiatul Aini, Nur Nur Muhammad Ansori, Arif Dhea Kharisma, Viol http://localhost:8080/xmlui/handle/123456789/9569 2025-01-07T07:54:32Z 2022-01-01T00:00:00Z

Title: Potential Roles of Purslane (Portulaca oleracea L.) as Antimetabolic Syndrome: A Review Authors: Sofiatul Aini, Nur; Nur Muhammad Ansori, Arif; Dhea Kharisma, Viol Abstract: The number of cases of obesity and type 2 diabetes mellitus (T2DM) is part of the metabolic syndrome case. Purslane (Portulaca oleracea L.) is a plant that has been clinically tested and has the potential to prevent and treat metabolic syndrome as well as pathogenic and pathophysiological activities that cause disease. The aim of this study is to discuss and conclude information regarding the activity and use of purslane (P. oleracea) as an antimetabolic. This review article is based on scientific publications found on Google Scholar and PubMed databases using the keywords of “Portulaca obesity”, “Portulaca overweight”, “Portulaca dyslipidemia”, and “Portulaca metabolic syndrome”. This plant acts on numerous pathways in the metabolic syndrome such as reduction of lipids, blood sugar, body weight and total cholesterol. Purslane (P. oleracea) can be used as a candidate for a new herbal plant as an anti-metabolic syndrome. Key words: Body weight, Portulaca oleracea, Insulin resistance, Metabolic syndrome, Obesity

2022-01-01T00:00:00Z Robbani, Sabila Elya, Berna Iswandana, Raditya http://localhost:8080/xmlui/handle/123456789/9568 2025-01-07T07:48:52Z 2022-01-01T00:00:00Z

Title: Alpha-glucosidase and DPP-IV Inhibitory Activities of Ethanol Extract from Caesalpinia sappan, Andrographis paniculata, and Syzygium cumini Authors: Robbani, Sabila; Elya, Berna; Iswandana, Raditya Abstract: Background: Diabetes is one of the fastest-growing global health problems of the 21st century. Antidiabetic medicine has been widely marketed with various mechanisms of action. However, there are side effects from these drugs. Therefore, most diabetic patients consume herbal as complementary. Plants that have been shown to have potential as an antidiabetic are Caesalpinia Sappan, Andrographis Paniculata and Syzygium Cumini. Objective: This study aims to examine the in vitro antidiabetic activity of single and combined ethanol extract of those three plants by inhibiting alpha-glucosidase and DPP-IV (Dipeptidyl peptidase IV) enzymes. Materials and Methods: The alpha-glucosidase inhibitory activity was determined using the paranitrofenil alfa-D-glukopiranosida (pNPG) reaction at a wavelength of 405 nm. Acarbose was used as the positive control. The DPP-IV inhibitory activity using H-Gly-Pro-AMC substrate and detected by fluorescence at λex = 365 nm and λem=415-445 nm. Sitagliptin was used as the positive control. LC-MS analysis was performed to identify the compounds contained in the combined extract. Results: Caesalpinia sappan showed better activity to inhibit alpha-glucosidase enzyme than acarbose at IC50 of 9,29 μg/mL. The combined extract obtained higher inhibition as DPP-IV inhibitor than single extract at 63, 69%. The highest compound in the combined extract were 5,7-Dihydroxy-3-(4'-hydroxybenzyl) chromone, Protosappanin E-1, Saurufuran B and candidate mass C36H38N4O5. Conclusion: These results indicate that single extract or combined extract potential as antidiabetic. Key words: Alpha-glucosidase inhibitor, Andrographis paniculata, Caesalpinia sappan, DPP-IV Inhibitor, Syzygium cumini

2022-01-01T00:00:00Z Veshalini K, Veshalini K http://localhost:8080/xmlui/handle/123456789/9567 2025-01-07T07:47:20Z 2022-01-01T00:00:00Z

Title: Phytochemical Screening, In vitro Antioxidant Activities and Zebrafish Embryotoxicity of Abelmoschus esculentus Extracts Authors: Veshalini K, Veshalini K Abstract: Background: Abelmoschus esculentus (L.) Moench (A. esculentus) commonly known as okra is being used as a medicinal plant traditionally, due to its phytochemical content that exhibits significant biological activities. Objective: The present study was undertaken to determine phytochemicals, antioxidant activity and embryotoxic effects of hexane extract (HE), chloroform extract (CE), methanol extract (ME), and aqueous extract (AE) of A. esculentus cultivated in Malaysia. Materials and Methods: A. esculentus extracts were screened for the phytochemicals while in vitro antioxidant activities were evaluated by performing 1,1-diphenyl-2-picrylhidrazyl (DPPH) assay and reducing power assay. Meanwhile, the embryotoxicity were accessed by exposing zebrafish embryos to the extracts and developmental endpoint recorded with median lethal concentration (LC50). Results: The phytochemical screening showed the presence of flavonoids, tannins, phenols, saponins, anthraquinones, alkaloids and reducing sugars. The highest phenolic content was obtained in the CE at 143.85 μg (GAE)/mg extract. Whereas the HE depicted the highest flavonoid content at 63.76 μg (QE)/mg extract. At 1000 μg/mL, ME showed the highest free radical scavenging activity at 28.86 % and reducing power at 0.271, in both DPPH and reducing power assay, respectively. While the CE presented a significant embryotoxic effect on zebrafish with the LC50 at 236.07 μg/mL. The morphological malformation of embryos, scoliosis and pericardial oedema were observed at 500 μg/mL of HE and ME treatment. Conclusion: As a result, with various ranges of phytochemical compounds, antioxidant capacities and lower toxic effects of A. esculentus extracts may be efficient in reducing the inflammation precursors that drive chronic inflammatory illnesses. Key words: Abelmoschus esculentus, Antioxidant capacity, Phytochemicals, Zebrafish embryotoxicity.

2022-01-01T00:00:00Z