http://localhost:8080/xmlui/handle/123456789/7727 2026-04-14T20:40:54Z 2026-04-14T20:40:54Z Albert, Tee Khiem, Pho Duc Beatrix, Musung Anastasia Dorothy, Wijaya Richard, Sutejo http://localhost:8080/xmlui/handle/123456789/7998 2024-11-16T03:13:34Z 2024-07-01T00:00:00Z

Title: The antiviral activity of Laportea decumana Methanolic extract against NDV virus Authors: Albert, Tee; Khiem, Pho Duc; Beatrix, Musung Anastasia; Dorothy, Wijaya; Richard, Sutejo Abstract: The avian species virus that causes Newcastle disease is an extremely contagious illness. Avian paramyxovirus 1, or Newcastle disease virus (NDV), is a virus that brings harm to poultry's central nervous system and digestive tract. The NDV outbreak was initially documented in 1928 in Java, Indonesia. Newcastle disease does not currently require therapy. One popular and useful strategy for preventing and treating viral infections, such as Newcastle disease, is vaccination. With a variety of native medicinal plants and an abundance of biodiversity, Indonesia presents a promising area for biotechnology and pharmaceutical research. Laportea decumana, is a native plant in the Eastern part of Indonesia that contains alkaloids, glycosides, steroids, flavonoids, tannin, and saponin. Its cytotoxic, analgesic, antioxidant, and antibacterial properties have all been demonstrated. The antiviral properties of L. decumana have not been extensively researched. Thus, the purpose of this study is to examine L. decumana's antiviral activity, particularly against NDV, using a variety of techniques, including the plaque assay, cytotoxicity test, and gene expression experiment. L. decumana extracts at 100 μg/mL or less is a safe concentration to consider, as it still has 65% and above cell viability based on the results of the cytotoxic assay.

2024-07-01T00:00:00Z Wardatun, Sri Sofihidayati, Trirakhma Afifah, Nida Juhroh, Juju http://localhost:8080/xmlui/handle/123456789/7997 2024-11-16T03:11:16Z 2024-07-01T00:00:00Z

Title: Ethanol-based solvent system for recovery antioxidant activity Centella asiatica L. Urban and its application in sleep-deprived Rats Authors: Wardatun, Sri; Sofihidayati, Trirakhma; Afifah, Nida; Juhroh, Juju Abstract: Antioxidants can eliminate free radicals by donating electrons to oxidants. Preclinical and clinical studies show that Centella asiatica L. Urban has antioxidant activity. Ethanol was the best solvent for extracting antioxidant compounds from Centella asiatica L. Urban. The concentration of ethanol solvents affects the compounds extracted and their antioxidant activities. The study used sleep-deprived rats with glutathione (GSH) concentration parameters to identify ethanol-based solvent systems for the optimum recovery of antioxidant activities and the in vivo antioxidant activity of the most active extract. C. asiatica L. Urban powder was macerated with 30, 50, 70, and 96% ethanol, respectively. Using a phosphomolybdate reagent, the extract's antioxidant activity was assessed. In vivo, antioxidant activity was tested on the extract with the highest in vitro antioxidant activity. Ethanol-based solvent systems show different recovery antioxidant activities of C. asiatica L. Urban. The antioxidant activity was most recovered in 70% ethanol, with an IC50 value of 76.76±25.29 µg/mL. Ethanol 70% C. asiatica L. Urban extract given to sleep-deprived rats at 300 mg/kg was not significantly different in glutathione levels from those group rats that received the positive control solution (vitamin C). The C. asiatica L. Urban 70% ethanol extract can increase glutathione levels in sleep-deprived rats.

2024-07-01T00:00:00Z Rusmalina, Siska Mahfur Hasanah, Nunung Wiyono, Mochamad Ardy Ekayanti, Nonik Nur Nathania, Jacinda Caroline http://localhost:8080/xmlui/handle/123456789/7996 2024-11-16T03:07:38Z 2024-07-01T00:00:00Z

Title: The optimation of fermentation time, antibacterial activity, and profiling secondary metabolite of symbiont fungi from Sponge Gelliodes fibulata Authors: Rusmalina, Siska; Mahfur; Hasanah, Nunung; Wiyono, Mochamad Ardy; Ekayanti, Nonik Nur; Nathania, Jacinda Caroline Abstract: Symbiont fungi are organisms that live in sponges tissue. Sponges are known to contain many metabolites which have the potential to be used as raw materials for medicine. Sponge Gelliodes fibulata belongs to the category demospongiae. The purpose of this study was to determine the optimal time to obtain the best secondary metabolite profile results in the sponge symbiont fungus Gelliodes fibulata. This research is included in experimental research. Beginning with the fungi culture of the sponge Gelliodes fibulata. Time variations 2, 4, 6, 8, 10, 12, and 14 are used to see differences in secondary metabolite production. A liquid extraction process is carried out to obtain secondary metabolites produced during fermentation. The final stage is to carry out qualitative analysis with TLC and antibacterial testing with the well-diffusion method. The results obtained indicate that the length of fermentation time influences the secondary metabolites obtained and automatically influences their antibacterial activity. The profile of secondary metabolites from TLC showed that the 10th day of fermentation had the secondary metabolites with high complexity and the highest yield 0.086%. The results of antibacterial activity showed that the 10th day of fermentation had the largest inhibition zone with 7.75 ± 0.44 mm compared to the other days of fermentation.

2024-07-01T00:00:00Z Sidharta, Boy Rahardjo Atmodjo, Patricius Kianto http://localhost:8080/xmlui/handle/123456789/7995 2024-11-16T03:02:33Z 2024-07-01T00:00:00Z

Title: Phytochemical and antibacterial analyses of essential oils extracted from the leaves of Euodia suaveolens Scheff Authors: Sidharta, Boy Rahardjo; Atmodjo, Patricius Kianto Abstract: Euodia suaveolens is one of the plants that ancient people in Indonesia used due to its manifold benefits. Earlier research on this plant was mostly done on its potency as a mosquito repellent. This present study aims to determine the phytochemical and antibacterial analyses of the essential oils (EOs) extracted from the leaves of E. suaveolens. The EOs of the leaves of E. suaveolens were extracted by steam distillation method and were analyzed phytochemically utilizing the GC-MS technique to determine the chemical constituents. The chemical components were tested on four pathogenic bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis utilizing the diffusion agar method. The results showed that the main compounds extracted from the EOs were α-curcumene, evodone, globulol, limonene, linalool, longipinenepoxide, menthofuran, and p-mentha-1,8-diene. The antibacterial analysis of these compounds showed potential activities to inhibit the growth of four pathogenic bacteria tested, but the inhibition zones formed were still lower compared to commercial antibiotic kanamycin. E. suaveolens EOs exhibited diameter of zone of inhibition as follows 2.03+0.22, 0.50+0.49, 1.38+0.10, 1.40+0.27 cm to E. coli, P. aeruginosa, S. aureus, and S. epidermidis while kanamycin showed 3.43+0.08, 3.25+0.08, 3.38+0.12, and 3.18+0.24 cm respectively. These results recommend that the main compounds extracted from the EOs of the leaves of E. suaveolens be explored further to determine their potencies as new antibiotic medications.

2024-07-01T00:00:00Z