http://localhost:8080/xmlui/handle/123456789/7300 2026-04-09T05:38:55Z 2026-04-09T05:38:55Z Amelia, Puteri Adila, Ersa Fiki Novitri, Suci Ahda Janatiningrum, Isra http://localhost:8080/xmlui/handle/123456789/7365 2024-11-06T08:07:58Z 2023-12-01T00:00:00Z

Title: Isolation of Escherichia coli bacteriophages from Domestic Wastewater in Tangerang Selatan Authors: Amelia, Puteri; Adila, Ersa Fiki; Novitri, Suci Ahda; Janatiningrum, Isra Abstract: In recent years, there has been a continuous increase in the prevalence of antibiotic resistance among bacteria responsible for infectious diseases. Several studies have shown that the occurrence of the COVID-19 pandemic is a major factor contributing to this rapid increase. To overcome this challenge, one promising approach is the use of bacteriophages as a treatment option. Therefore, this study aims to isolate E. coli lytic bacteriophages as an alternative antibiotic for treating secondary bacterial infections of COVID-19. Isolation of bacteriophages was carried out using the plaque assay method with double layer agar technique. The isolate obtained was purified and tested for effectiveness in lysing E. coli ATCC 25922 bacterial cells. The results showed that E. coli had resistance to antibiotics, such as Amoxicillin, Clindamycin, Ciprofloxacin, Azithromycin, and Trimethoprim/sulfamethoxazole. BEB1 isolate obtained from domestic waste had activity in inhibiting the growth of the test bacteria with an effectiveness of 72% at the 25th hour. Based on these findings, it had the potential to be developed as an alternative antibiotic agent to overcome infectious diseases caused by E. coli ATCC 25922. In recent years, there has been a continuous increase in the prevalence of antibiotic resistance among bacteria responsible for infectious diseases. Several studies have shown that the occurrence of the COVID-19 pandemic is a major factor contributing to this rapid increase. To overcome this challenge, one promising approach is the use of bacteriophages as a treatment option. Therefore, this study aims to isolate E. coli lytic bacteriophages as an alternative antibiotic for treating secondary bacterial infections of COVID-19. Isolation of bacteriophages was carried out using the plaque assay method with double layer agar technique. The isolate obtained was purified and tested for effectiveness in lysing E. coli ATCC 25922 bacterial cells. The results showed that E. coli had resistance to antibiotics, such as Amoxicillin, Clindamycin, Ciprofloxacin, Azithromycin, and Trimethoprim/sulfamethoxazole. BEB1 isolate obtained from domestic waste had activity in inhibiting the growth of the test bacteria with an effectiveness of 72% at the 25th hour. Based on these findings, it had the potential to be developed as an alternative antibiotic agent to overcome infectious diseases caused by E. coli ATCC 25922.

2023-12-01T00:00:00Z Rasdianah, Nur Akuba, Juliyanty Djuwarno, Endah Nurrohwinta http://localhost:8080/xmlui/handle/123456789/7362 2024-11-06T08:01:32Z 2023-12-01T00:00:00Z

Title: Knowledge and Beliefs about the Use of Antibiotics in Society:A Questionnaire Study of Gorontalo Province, Indonesia Authors: Rasdianah, Nur; Akuba, Juliyanty; Djuwarno, Endah Nurrohwinta Abstract: A lack of public understanding of the use of antibiotics has the potential to cause inappropriate use of antibiotics. The research aims to obtain information about the knowledge and beliefs of respondents regarding the use of antibiotics in Gorontalo Province. The research was conducted in various public places in four regions in Gorontalo Province with a population of 907,389 people. Sampling was carried out randomly (n=384), with inclusion criteria namely being over 18 years old, having used antibiotics in the last year, and being willing to take part in the research. Respondents who were unable to communicate in Indonesian were excluded from this study. The instruments used were demographic characteristics data collection sheets, knowledge and belief questionnaires.ata was collected using a questionnaire and the results were analyzed using descriptive statistics. Of the 384 respondents, 55.99% of respondents knew that antibiotics must be purchased with a doctor's prescription and that various types of antibiotics can be used for one type of bacterial infection (70.31%), antibiotics could be taken twice at the next dose (63.80%), accelerated recovery (60.16%), its use was stopped when symptoms disappeared (56.25%), the method of use was the same as other drugs (52.86) and effectively treated viruses (51.82). %), antibiotics can cause allergies (52.34%), resistance (59.38%), and bacterial resistance can increase if their use is not appropriate (48.96%). The results of the correlation analysis showed that there was a significant and unidirectional relationship between the two (rho=0.467). Respondents have adequate knowledge about antibiotics, resistance, and side effects but have inaccurate knowledge about the effectiveness of antibiotics for viral infections. Respondents have sufficient confidence that antibiotics cannot be obtained without a prescription, do not treat all diseases, and can cause resistance and side effects.

2023-12-01T00:00:00Z Fitriani, Lili Simbolon, Clara Alverina Zaini, Erizal http://localhost:8080/xmlui/handle/123456789/7359 2024-11-06T07:54:05Z 2023-12-01T00:00:00Z

Title: Preparation and Characterization of Multicomponent Crystal Piperine-Caffeine Authors: Fitriani, Lili; Simbolon, Clara Alverina; Zaini, Erizal Abstract: Piperine (C17 H19 NO3 ) belongs to the alkaloid group mainly found in the Piperaceae family, especially in black pepper (Piper nigrum L.). Piperine is practically insoluble in water. This study aims to improve the solubility of piperine by preparing it into a multicomponent crystal using caffeine as a coformer. The formation of piperine-caffeine multicomponent was prepared by solvent evaporation and solvent drop grinding. Each sample was characterized using Powder X-ray diffraction (XRD), Differential Scanning Calorimetry (DSC), and FT-IR spectroscopy. The solubility test was carried out by sonication for 30 minutes at room temperature (30 ⁰C), and the dissolved piperine was analyzed by High-Performance Liquid Chromatography (HPLC) using methanol: water (80:20) as the mobile phase. This study shows a decrease in peak diffraction intensity on PXRD analysis and a reduction in endothermic peak on the DSC thermogram of multicomponent crystals. In the FTIR analysis, multicomponent crystals slightly shift, indicating the physical interaction. The multicomponent crystal of piperine-caffeine prepared by solvent evaporation method has an increase in solubility of 2.56 times, and the multicomponent crystal with solvent drop grinding method has an increase in solubility of 1.61 times, respectively. In conclusion, the multicomponent crystal piperine–caffeine can enhance the solubility of piperine.

2023-12-01T00:00:00Z Syafni, Nova Fakhriadi, Athallah Nofrizal Ismed, Friardi Putra, Deddi Prima http://localhost:8080/xmlui/handle/123456789/7357 2024-11-06T07:43:26Z 2023-12-01T00:00:00Z

Title: Inhibition of Edible Plant Torch Ginger (Etlingera elatior (Jack) R. M. Sm.) against α-Glucosidase and α-Amylase Authors: Syafni, Nova; Fakhriadi, Athallah; Nofrizal; Ismed, Friardi; Putra, Deddi Prima Abstract: Indonesia's diabetes cases were in the top ten list globally, with 90% of the patients being type-2 diabetes mellitus (T2DM). An approach for screening the local edible plants is made for managing or treating T2DM by inhibiting α-glucosidase and α-amylase enzymes. Results showed that inflorescence of Etlingera elatior inhibition toward both enzymes. TLC-autography and IC50 value of fractions from E. elatior were used to identify the potential fractions and possible compounds for the activity. The non-polar fraction was spotted in the active substances based on TLC-autography. Then, the targeted compounds were separated by column chromatography to obtain stigmasterol as one of the active compounds. The IC50 values of total extract, n-hexane, EtOAc, n-BuOH, and isolated compound against α-glucosidase were 16.0, 7.5, 13.5, 9.7, and 2.0 ppm, respectively. The IC50 values for α-amylase inhibition were respectively 88.6, 48.6, 23.2, 29.1, and 27.5 ppm. The positive control (acarbose) against α-glucosidase and amylase exhibited IC50 values of 153.2 and 12.3 ppm. The inhibition of E. elatior against the two enzymes could be an alternative to delay carbohydrate absorption.

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