http://localhost:8080/xmlui/handle/123456789/6720 2026-04-14T20:30:00Z 2026-04-14T20:30:00Z Fahim Shahriar, Md Kabir, Janisa Piaopiao, Ding http://localhost:8080/xmlui/handle/123456789/6743 2024-10-26T02:44:09Z 2023-01-01T00:00:00Z

Title: Dielectrophoresis spectroscopy for nucleotide identification in DNA Authors: Fahim Shahriar, Md; Kabir, Janisa; Piaopiao, Ding Abstract: Dielectrophoresis spectroscopy for nucleotide identification in DNA Md Fahim Shahriar a,*, Janisa Kabir b, Ding Piaopiao c a School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China b Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing, 210009, China c School of Pharmacy, Hubei University of Science and Technology, Hubei, China A R T I C L E I N F O Keywords: Spectroscopy Single nucleotide polymorphism Microarrays Dielectrophoresis Oligonucleotide A B S T R A C T DNA sequence with a known physical position on a chromosome is called a genetic marker, so the causal gene may identify with genetic markers in different kinds of hereditary diseases. DNA segments near one another on a chromosome often inherit the other concurrently; as a result, the inheritance of a neighboring gene that has not yet been discovered but whose general position is tracked by using genetic markers. So, Genetic markers can play a significant role in biological research because they can contribute to identifying many diseases. Single nucleotide polymorphism, or SNP (pronounced “snip”), is the variation of a single nucleotide in a DNA due to genetic disorders. For example, in a specific region of DNA, an SNP may displace the nucleotide cytosine (C) with the nucleotide thymine (T). SNPs, or single nucleotide polymorphisms, are one of the most common genetic variations that assist in detecting many human diseases such as Migraine, Cancer, Schizophrenia, Sickle Cell Anemia, Alzheimer's Disease, etc. Hyperchromicity, Short Oligonucleotide Analysis Program (SOAP), quantitative PCR techniques, Fluorescence Polarization Melting Curve Analysis, SNP Microarrays, Intercalating Dyes, and many other techniques are commonly used to identify SNPs nowadays. However, those methods are not much reliable, a bit costly, time-consuming, and difficult to use, whereas dielectrophoresis can be an excellent way to detect SNP easily. A non-uniform electric field generated by electrodes interacts with polarizable suspended particles to regulate and alter particle movement; this process is known as dielectrophoresis (DEP). Cell transfer, in vitro fertilization, and biological testing are a few uses for dielectrophoresis, particularly in the biomedical industry. Cell fusion using dielectrophoresis has also improved crossbreeding, cancer treatment, and scientific research. Most notably, dielectrophoresis is used to classify changes in the electrical characteristics of cells. In this phenomenon, when a dielectric particle is exposed to a non-uniform electric field, a force is produced on it, and this DEP force may be utilized to recognize the variations in a single location in a DNA sequence. DEP is less timeconsuming, cheap, and reliable than other processes to detect SNPs easily.

2023-01-01T00:00:00Z Mori, Antonio Lavezzari, Denise Pomari, Elena Deiana, Michela Piubelli, Chiara Rosaria Capobianchi, Maria Castilletti, Concetta http://localhost:8080/xmlui/handle/123456789/6740 2024-10-26T02:41:12Z 2023-01-01T00:00:00Z

Title: sgRNAs: A SARS-CoV-2 emerging issue Authors: Mori, Antonio; Lavezzari, Denise; Pomari, Elena; Deiana, Michela; Piubelli, Chiara; Rosaria Capobianchi, Maria; Castilletti, Concetta Abstract: sgRNAs: A SARS-CoV-2 emerging issue Antonio Mori 1, Denise Lavezzari 1, Elena Pomari, Michela Deiana, Chiara Piubelli, Maria Rosaria Capobianchi *, Concetta Castilletti Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024, Verona, Italy A R T I C L E I N F O Handling Editor: Prof A Angelo Azzi Keywords: sgRNAs SARS-CoV2 Bioinformatics Next-generation sequencing Viruses Coronavirus A B S T R A C T Like for other coronaviruses, SARS-CoV-2 gene expression strategy is based on the synthesis of a nested set of subgenomic mRNA species (sgRNAs). These sgRNA are synthesized using a “discontinuous transcription” mechanism that relies on template switching at Transcription Regulatory Sequences (TRS). Both canonical (csgRNA) and non-canonical (nc-sgRNA, less numerous) subgenomic RNA species can be produced. Currently, sgRNAs are investigated on the basis of sequence data obtained through next generation sequencing (NGS), and bioinformatic tools are crucial for their identification, characterization and quantification. To date, few software have been developed to this aim, whose reliability and applicability to all the available NGS platforms need to be established, to build confidence on the information resulting from such tools. In fact, these information may be crucial for the in depth elucidation of viral expression strategy, particularly in respect of the significance of ncsgRNAs, and for the possible use of sgRNAs as potential markers of virus replicative activity in infected patients.

2023-01-01T00:00:00Z Zakrzewska, K. Arvia, R. Bua, G. Margheri, F. Gallinella, G. http://localhost:8080/xmlui/handle/123456789/6738 2024-10-26T02:36:14Z 2023-01-01T00:00:00Z

Title: Parvovirus B19: Insights and implication for pathogenesis, prevention and therapy Authors: Zakrzewska, K.; Arvia, R.; Bua, G.; Margheri, F.; Gallinella, G.

2023-01-01T00:00:00Z Giorgio Spezia, Pietro Focosi, Daniele Baj, Andreina Novazzi, Federica Drago Ferrante, Francesca Carletti, Fabrizio Minosse, Claudia Matusali, Giulia Maggi, Fabrizio http://localhost:8080/xmlui/handle/123456789/6734 2024-10-26T02:21:35Z 2023-01-01T00:00:00Z

Title: TTV and other anelloviruses: The astonishingly wide spread of a viral infection Authors: Giorgio Spezia, Pietro; Focosi, Daniele; Baj, Andreina; Novazzi, Federica; Drago Ferrante, Francesca; Carletti, Fabrizio; Minosse, Claudia; Matusali, Giulia; Maggi, Fabrizio Abstract: TTV and other anelloviruses: The astonishingly wide spread of a viral infection Pietro Giorgio Spezia a, Daniele Focosi b, Andreina Baj c, Federica Novazzi c, Francesca Drago Ferrante c, Fabrizio Carletti d, Claudia Minosse d, Giulia Matusali d, Fabrizio Maggi d,* a Department of Translational Research, University of Pisa, Italy b North-Western Tuscany Blood Bank, Pisa University Hospital, Pisa, Italy c Department of Medicine and Surgery, University of Insubria, Varese, Italy d Laboratory of Virology and Biosafety Laboratories, National Institute of Infectious Diseases “L. Spallanzani” IRCCS, Rome, Italy A R T I C L E I N F O Handling Editor: Prof A Angelo Azzi Keywords: TTV Anellovirus TTMV TTMDV Virome Immune system A B S T R A C T The broad family of viruses known as anelloviruses (AV) infects both humans and numerous animal species. They have a tiny, covalently closed single-stranded DNA genome and the astonishing capacity to infect a very high percentage of healthy and ill people with chronic infections that could last a lifetime. AV, and particularly the prototype Torquetenovirus, have established a successful interaction with the host's immune system and the rate at which they replicate is a gauge to measure overall immune function, even though many aspects of their life cycle and pathogenesis are still poorly understood.

2023-01-01T00:00:00Z