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Gene expression of selected apoptotic markers in human oral Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract

2023-02-10T04:34:06Z

Title: Gene expression of selected apoptotic markers in human oral Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract Authors: Lestari, Widya; N A W Yusry, Wan; H. Iskandar, Siti; J A Ichwan, Solachuddin; I. Irfan, Nining Abstract: Geneexpressionofselectedapoptoticmarkersinhumanoralsquamous carcinomaHSC-3celllinetreatedwithMyrmecodiapendansplantextract WidyaLestari1*,WanNAWYusry1,SitiHIskandar1,SolachuddinJAIchwan1,NiningIIrfan2, WastutiHSuriyah3 1. KulliyyahofDentistry,InternationalIslamicUniversityMalaysia,Pahang25200,Malaysia 2. InternationalInstituteofHalalResearchandTraining,InternationalIslamicUniversityMalaysia, Selangor53100,Malaysia 3. KulliyyahofPharmacy,InternationalIslamicUniversityMalaysia,Pahang25200,Malaysia *E-mail:drwidya@iium.edu.my Abstract Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study aimed to investigate the effect of M. pendans treated samples on the expression of apoptotic markers, Bax and Bcl-2. Methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was dried thoroughly and then extractedaqueously.Theapoptoticactivitywasdetectedviaflowcytometry.BaxandBcl-2expressionwasanalyzedby quantitative real-time polymerase chain reaction. Results: Results of our cell cycle analysis reveal that aqueous extract of M. pendans induced apoptosis in 2.5 and 5 mg/mL but no change between these two concentrations. Apoptosis was observed at 24 h but not at 48 h. Bax and Bcl-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, upregulation of Bax was observed at 2.5 mg/mL. However, after 48 h, Bax expression showed no changes at any concentration. Bcl-2 was significantlydownregulated after 48 h of treatment. Conclusions: M. pendans extract induced apoptosisinHSC-3cells,whichmightoccurviatheproapoptotic(Bax)andantiapoptotic(Bcl-2)pathways. Keywords: apoptosis,cellcycle,flowcytometry,real-timepolymerasechainreaction

Theeffectofsappanwoodextractsintreatingdiabetesinducedinmice

2023-02-10T04:27:10Z

Title: Theeffectofsappanwoodextractsintreatingdiabetesinducedinmice Authors: AISakir, Noviana; GKim, Jae Abstract: Theeffectofsappanwoodextractsintreatingdiabetesinducedinmice NovianaAISakir*,JaeGKim DepartmentofBiologyEducation,SeoulNationalUniversity,Seoul08826,SouthKorea *E-mail:novianaastutiirnasakir@gmail.com Abstract Background: Since 2015, an estimated 415 million people had diabetes worldwide. Although, there is no scientific research related to the effectiveness of chemical substance (brazilin) in Caesalpinia sappan L. that can decrease blood glucoselevelin humans, manypeopleinSulawesiconsume this wood for diabetestreatment.Thisstudyaimed to prove the effect of sappan wood extract on decreasing blood glucose levels in mice and to identify the most effective dose. Methods: Experimental research (pretest and posttest randomized controlled group design) was conducted on 20 male albino mice [body weight (bw): 20–30 g] used as alloxan-induced diabetic models and were divided into four treatment groups according to alloxan dose: control and 0.25, 0.50, and 0.75 g/kg bw (n = 5 for all groups) groups. Results: Significanteffectsofsappanwoodextractondecreasingbloodglucoselevelsinmicewerenotedinthepretestandposttest (pvaluesare0.754and0.901respectively).Conclusions:Sappanwoodextractcouldreducebloodglucoselevelsinmice with diabetes induced by alloxan at 0.25, 0.50, and 0.75 g/kg bw. The extract with 0.50 g/kg bw dose was the most effectiveindecreasingglucoselevelsinhyperglycemicmice. Keywords:antihyperglycemic,diabetesmellitus,heartwood

Protection against neutrophil extracellular trap (NET) toxicity by Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER

2023-02-10T03:22:02Z

Title: Protection against neutrophil extracellular trap (NET) toxicity by Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER Authors: Astuti, Puji; M A Beurskens, Danielle; Vajen, Tanja; A F Nicolaes, Gerry; Zhang, Ming Abstract: Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER Puji Astuti1,2*, Danielle M A Beurskens3, Tanja Vajen4, Gerry A F Nicolaes3*, Ming Zhang1, Guido R M M Haenen1 1. Department of Pharmacology and Toxicology, Faculty of Health, Medicine, and Life Science, Maastricht University, 6229 ER Maastricht, The Netherlands 2. Polytechnic 'Aisyiyah Pontianak, Pontianak 78116, Indonesia 3. Department of Biochemistry, Faculty of Health, Medicine, and Life Science, Maastricht University, 6229 ER Maastricht, The Netherlands 4. Department of Biochemistry, Faculty of Health, Medicine, and Life Science, Maastricht University, 6229 ER Maastricht, The Netherlands *E-mail: pujiii.astutiii@gmail.com Abstract Background: Neutrophil extracellular traps (NET) are extracellular fibers produced by activated neutrophils to kill bacteria. NET was recently found to be associated with several diseases, such as autoimmune diseases. NET formation, called NETosis, is reactive oxygen species (ROS) dependent, thereby, prompting us to study its inhibition by potent antioxidant monoHER as well as to study monoHER protection against NET toxicity caused by NET constituent histone 3 on endothelial cells. Methods: Freshly isolated neutrophils from male donors were stimulated with PMA to induce NET formation. The effect of monoHER (50 µM) on oxidative burst (O2●− production) and NET formation was determined by fluorescence microscopy. Flow cytometry was used to determine the protective effect of monoHER against NET toxicity constituent histone 3 in EA.hy926 cells. Data was evaluated using ANOVA followed by the Bonferroni post-hoc test. Results: MonoHER significantly reduced (p < 0.01) O2●− production of PMA-stimulated neutrophils and consequently inhibited NET formation. MonoHER could also counteract histone 3 toxicity in EA.hy926 cells. Conclusions: MonoHER might inhibit ROS-dependent NETosis pathway and also protect the endothelial cells against NET toxicity. Keywords: antioxidants, extracellular matrix proteins, flavonoids, monoHER, neutrophils, protective agents

Effect of coral Goniopora Sp scaffold application on human Effect of coral Goniopora Sp scaffold application on human osteoblast-like MG-63 cell activity in vitro

2023-02-09T07:51:49Z

Title: Effect of coral Goniopora Sp scaffold application on human Effect of coral Goniopora Sp scaffold application on human osteoblast-like MG-63 cell activity in vitro Authors: Julia, Vera; Abbas, Basril; W. Bachtia, Endang; S. Latief, Benny; M. Kuijpers-Jagtman, Anne Abstract: Effect of coral Goniopora Sp scaffold application on human osteoblast-like MG63 cell activity in vitro Vera Julia1*, Basril Abbas2, Endang W Bachtiar3, Benny S Latief1, Anne M Kuijpers-Jagtman4 1. Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia 2. National Nuclear Energy Agency of Indonesia, Jakarta 12710, Indonesia 3. Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia 4. Department of Dentistry, Section of Orthodontics and Craniofacial Biology, Radboud University Medical Centre, 6500 HB Nijmegen, the Netherlands *E-mail: drgverajuliaspbm@gmail.com Abstract Background: Coral is an osteo-conductive biomaterial that can act as an alternative scaffold for osteogenesis. In this in vitro study we analyzed the activity of osteoblast-like cells after treatment with the coral Goniopora. Methods: Human osteoblast-like MG-63 cells were incubated in α-minimal essential medium supplemented with 10% fetal bovine serum and 300 ng/mL amphotericin B plus 1% penicillin-streptomycin and stored in a 5% CO2 incubator at 37°C. The Goniopora were smashed into size A (20 mesh), B (1–2 mm), and C (200 mesh) particles, sterilized using gamma radiation and applied to cells. Protein and alkaline phosphatase (ALP) concentrations were evaluated after incubation for 24 and 48 h. Results: The protein assay of 24 h and 48 h cultured osteoblasts illustrated that treated cells, whether with coral size A, B and C exhibited a lower mean value compared to the untreated cells. For ALP levels there were statistically significant differences at 48 h between B and C (p = 0.004), and A and C (p = 0.09). Conclusions: No significant differences in total protein concentrations were found among all groups after 24 and 48 h. Smaller coral size and longer incubation time tended to facilitate osteogenesis. These results require further empirical validation. Keywords: alkaline phosphatase, biocompatible materials, bone regeneration, bone substitutes, coral, osteoblasts