http://localhost:8080/xmlui/handle/123456789/3772 2026-04-29T06:39:27Z 2026-04-29T06:39:27Z Mahwati, Yeni http://localhost:8080/xmlui/handle/123456789/3787 2023-01-27T07:55:25Z 2019-01-01T00:00:00Z

Title: Effect of body weight changes on hypertension in Indonesian adults (A 14-year follow up) Authors: Mahwati, Yeni Abstract: Effect of body weight changes on hypertension in Indonesian adults (A 14-year follow up) Yeni Mahwati Public Health, Health Institute of Dharma Husada, Antapani, Bandung 40282, Indonesia E-mail: yenimahwati@stikesdhb.ac.id Abstract Background: Obesity is one of the major risk factors of hypertension. There is no large cohort study designed to investigate the quantitative association between body weight changes and the risk of hypertension in Indonesia. The aim of this study was to determine the impact of longitudinal BMI changes on hypertension in Indonesian adults. Methods: Body Mass Index (BMI) was computed by dividing weight (kg) by height squared (m2). Based on the BMI at baseline, the participants formed four weight-change groups: normal weight-maintainers, weight-gainers, weight-losers and overweight or obese-maintainers. The effect of age on the relationship between body weight changes and hypertension was analyzed by logistic regression models using stratified analysis. Results: Four body weight changes were identified: normal weight-maintainers (41.95%), weight-gainers (18.83%), weight-losers (5.24%), and overweight or obesemaintainers (33.98%). The stratified logistic regression analysis showed that changes in the relationships between the BMI changes and hypertension with age generally tended to be positive in the younger age-based subgroups but negative in the older subgroups. Relative to the normal weight-maintainers, the weight-gainers had the highest likelihood of hypertension (OR=1.68 95%CI [1.23-1.93]). Conclusions: The findings of the study underline the importance of maintaining normal weight for preventing hypertension especially for the middle-aged. Keywords: body mass index, body weight changes, longitudinal,hypertension

2019-01-01T00:00:00Z H. Tacir, Ibrahim Z. Ulku, Sabiha A. Ketani, Muzaffer Z. Akdag, Mehmet http://localhost:8080/xmlui/handle/123456789/3786 2023-01-27T07:49:38Z 2019-01-01T00:00:00Z

Title: Histological changes to the rat mandibular condyle in response to long-term exposure to an extremely low frequency magnetic field Authors: H. Tacir, Ibrahim; Z. Ulku, Sabiha; A. Ketani, Muzaffer; Z. Akdag, Mehmet Abstract: Histological changes to the rat mandibular condyle in response to long-term exposure to an extremely low frequency magnetic field Ibrahim H Tacir1*, Sabiha Z Ulku1, Muzaffer A Ketani2, Mehmet Z Akdag3 1. Department of Prosthetic Dentistry, Faculty of Dentistry, Dicle Unıversity, Diyarbakır 21280, Turkey 2. Department of Histology, Medical Veterinary Faculty, Dicle University, Diyarbakır 21280, Turkey 3. Department of Biophysics, Medical Faculty, Dicle Unıversity, Diyarbakır 21280, Turkey *E-mail: ihtcr@hotmail.com Abstract Background: The need for electrical power continues to increase along with the rapidly growing economies of industrialized countries. However, the effects of electrical fields (EFs) on human health remain unclear. Hence, the aim of the present study is to evaluate the histological effects of an extremely low frequency magnetic field (ELF-MF) on the rat mandibular condyle. Methods: Twenty female Sprague–Dawley rats were randomly allocated to one of two groups (n = 10): an untreated control group and an ELF-MF group exposed to a 1.5-mT ELF-MF for 4 h per day for 6 months. After exposure, the left mandibular condyles of all rats were collected for histopathological examinations. Results: The histopathological changes to the ELF-MF group included a non-homogenous appearance and decreased thickness of the hypertrophic layer, irregular appearances of bone marrow cavities, significant deterioration, and delayed calcification and ossification. There were no significant differences in the thicknesses or histomorphometric appearances of the fibrotic, proliferative, and hypertrophic layers of the mandibular condyle of rats between the groups (p = 0.05). Conclusion: Long-term ELF-MF exposure can induce histopathological changes to the mandibular condyle of rats. Keywords: bone, magnetic field, mandibular condyle, rats

2019-01-01T00:00:00Z W. Dinutanayo, Wimba T. Keilholz, Ingeborg Braunholz, Diana http://localhost:8080/xmlui/handle/123456789/3785 2023-01-27T07:45:55Z 2019-01-01T00:00:00Z

Title: Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cells Authors: W. Dinutanayo, Wimba; T. Keilholz, Ingeborg; Braunholz, Diana Abstract: Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cells Wimba W Dinutanayo1*, Ingeborg T Keilholz2, Diana Braunholz2 1. Faculty of Health, Medicine, and Life Science, Maastricht University, 6200 MD Maastricht, the Netherlands 2. Translational Radiation Oncology Research Laboratory, Department of Radiation Oncology and Radiotherapy, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany *E-mail: wimba.dinutanayo@gmail.com Abstract Background: CTCs are present only in small numbers in patients’ blood. This study aimed to establish a protocol for enumeration and phenotypic characterization of circulating tumor cells (CTCs) by ImageStreamX MK II (AMNIS) imaging flow cytometry and to characterize the expression of epithelial, mesenchymal, and stem cell markers in CTCs. Methods: The study used the FaDu cell line at different passages, cisplatin-resistant (FaDu CDDP-R), and irradiationresistant (FaDu IR-R) subclones, as well as blood samples from head and neck squamous cell carcinoma (HNSCC) and colorectal cancer (CRC) patients for CTC detection (n = 5). Cells were fixed using 4% paraformaldehyde and permeabilized by incubation in 0.3% Triton X-100. The cell suspensions were stained with 1:100 EpCAM AF-488, 1:50 CD45 AF-647, 1:50 Vimentin AF-555, and 1:50 ALDH1A AF-594 antibodies. Results: There were significant differences in EpCAM expression levels between FaDu at late passage and FaDu CDDP-R subclones, as well as between FaDu at late passage compared with FaDu IR-R. Furthermore, CTCs were successfully detected in five patients’ samples with various CTC subpopulations. Conclusions: Intratumor heterogeneity in CTC phenotypes existed in CRC and HNSCC. Furthermore, three main subpopulations of CTCs were detected. Our findings strongly support future phenotypic studies of CTCs. Keywords: cancer stem cell, circulating tumor cell, epithelial-mesenchymal transition, imaging flow cytometry, liquid biopsy, neoplastic cells

2019-01-01T00:00:00Z H. Suriyah, Wastuti J A Ichwan, Solachuddin R. Kasmuri, Abdul Taher, Muhammad http://localhost:8080/xmlui/handle/123456789/3784 2023-01-27T07:37:55Z 2019-01-01T00:00:00Z

Title: In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line Authors: H. Suriyah, Wastuti; J A Ichwan, Solachuddin; R. Kasmuri, Abdul; Taher, Muhammad Abstract: In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line Wastuti H Suriyah1, Solachuddin J A Ichwan2*, Abdul R Kasmuri1, Muhammad Taher3 1. Department of Basic Medical Sciences, International Islamic University Malaysia, Pahang 25200, Malaysia 2. Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry, International Islamic University Malaysia, Pahang 25200, Malaysia 3. Department of Pharmaceutical Technology Kulliyyah of Pharmacy, International Islamic University Malaysia, Pahang 25200, Malaysia *E-mail: solachuddin@iium.edu.my Abstract Background: Type 2 diabetes mellitus is a chronic metabolic disorder that is associated with mortality and morbidity. Recently, several plant-based agents have been used in the management of diabetes. Pluchea indica has been traditionally consumed as a medicinal plant in Southeast Asia, and its leaves have demonstrated induction of hypoglycemic effect in normal rats. This in vitro study aimed to evaluate the potency of P. indica extracts in stimulating glucose consumption in human liver CCL-13 cell line model. Methods: P. indica leaves were dried and extracted using a series of organic solvents and water. The effect of the extracts on cell viability was determined by MTT assay. The glucose consumption was analyzed using glucose oxidase method. Results: Our results revealed that the methanol extract of P. indica could significantly increase glucose consumption of cells in a concentration-dependent manner, which suggests the usefulness of the extract as an antidiabetic candidate via stimulation of glucose uptake into the liver cells. Conclusion: Our study suggests that P. indica is a potential natural candidate for diabetes mellitus management. Keywords: diabetes mellitus type 2, liver, cell line, glucose oxidase, Pluchea indica

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