DSpace Collection: 281 - 382281 - 382http://localhost:8080/xmlui/handle/123456789/121192026-04-15T15:10:10Z2026-04-15T15:10:10ZUnusual Helicobacter pylori bacteremia in an immunocompetent elderly presenting as right ankle cellulitishttp://localhost:8080/xmlui/handle/123456789/122242025-07-19T02:44:51Z2025-02-01T00:00:00ZTitle: Unusual Helicobacter pylori bacteremia in an immunocompetent elderly presenting as right ankle cellulitis2025-02-01T00:00:00ZCytomegalovirus-related thrombocytopenia in two immunocompetent siblings following vertical transmission through Breast milkhttp://localhost:8080/xmlui/handle/123456789/122232025-07-19T02:43:59Z2025-01-01T00:00:00ZTitle: Cytomegalovirus-related thrombocytopenia in two immunocompetent siblings following vertical transmission through Breast milk2025-01-01T00:00:00ZLife-threatening COVID-19 in a thymoma patient with anti-interferon-α autoantibodiesShih, Yu-ShanTsai, Wan-TingTsai, Wan-TingGuo, Bei-ChiaCheng, AristineWang, Jann-Tayhttp://localhost:8080/xmlui/handle/123456789/122222025-07-19T02:42:16Z2025-02-01T00:00:00ZTitle: Life-threatening COVID-19 in a thymoma patient with anti-interferon-α autoantibodies
Authors: Shih, Yu-Shan; Tsai, Wan-Ting; Tsai, Wan-Ting; Guo, Bei-Chia; Cheng, Aristine; Wang, Jann-Tay
Abstract: Neutralizing anti-interferon (IFN)-α autoantibodies can lead to immune dysregulation, potentially resulting in
critical coronavirus disease 2019 (COVID-19). We report a case presenting with severe COVID-19 who was
subsequently diagnosed to have thymoma and neutralizing anti-IFN-α autoantibodies.2025-02-01T00:00:00ZPerformance of 30 protocol combinations for the detection of Cryptosporidium parvum in stool samplesBailly, EloïseBaranton, Chlo´eValot, St´ephaneVincent, AnneBegue, Herv´eBeclere, Corentinhttp://localhost:8080/xmlui/handle/123456789/122212025-07-19T02:39:20Z2025-01-01T00:00:00ZTitle: Performance of 30 protocol combinations for the detection of Cryptosporidium parvum in stool samples
Authors: Bailly, Eloïse; Baranton, Chlo´e; Valot, St´ephane; Vincent, Anne; Begue, Herv´e; Beclere, Corentin
Abstract: Background: Intestinal parasitic diseases affect millions of people worldwide. Numerous commercial molecular
methods detecting digestive parasites have been developed recently, including multiplex PCR assays able to
identify multiple parasites at once. Several studies have demonstrated that the efficacy of these molecular
methods is dependent on the specific protocols employed at each stage of the process including pretreatment,
extraction and amplification. However, previous studies have exclusively focused on one of these steps, without
considering the others. The objective of the present study was to evaluate the performances of molecular tools for
Cryptosporidium parvum detection in stool samples, considering all steps of the process simultaneously.
Methods: 30 distinct combinations of protocols were evaluated corresponding to three pre-treatment methods,
four DNA extraction techniques and six DNA amplification assays. The performances of these combinations were
evaluated in terms of detection limit.
Results: We showed that different combinations yielded varying results. The FTD® Stool Parasite technique
proved to be the most effective, achieving 100 % detection. Manual extraction methods demonstrated excellent
outcomes, although they are time-consuming. The optimal approach for detecting C. parvum DNA is a combination of mechanical pretreatment, the Nuclisens® Easymag® extraction method, and the FTD® Stool Parasite
DNA amplification method.
Conclusion: This work shows that the molecular diagnosis should consider all stages. A PCR method may not be
effective with an unsuitable extraction technique, but can yield optimal results with an appropriate one.2025-01-01T00:00:00Z